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首页> 外文期刊>Journal of Parasitology >AMPLIFICATION OF TRYPANOSOMA CRUZI–SPECIFIC DNA SEQUENCES IN FORMALIN-FIXED RACCOON TISSUES USING POLYMERASE CHAIN REACTION
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AMPLIFICATION OF TRYPANOSOMA CRUZI–SPECIFIC DNA SEQUENCES IN FORMALIN-FIXED RACCOON TISSUES USING POLYMERASE CHAIN REACTION

机译：聚合酶链反应扩增福尔马林固定的浣熊组织中特氏锥虫特异的DNA序列

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摘要

This investigation applied polymerase chain reaction (PCR) using 3 sets of Trypanosoma cruzi–specific primers to amplify DNA from 31 archived formalin-fixed and fresh-frozen raccoon hearts. PCR successfully amplified T. cruzi–specific sequences, with at least 1 primer set, from multiple sites within the myocardium of formalin-fixed and fresh-frozen raccoon hearts that had previously tested positive using enzyme-linked immunosorbent assay and indirect immunofluorescent antibody titer in the absence of positive hemoculture results. Trypanosoma cruzi DNA was most frequently amplified from the interventricular septum, right ventricle, and left atrium. In addition, T. cruzi DNA was amplified with all 3 primers in at least 1 raccoon that was hemoculture positive and 2 animals that were borderline negative for the T. cruzi antibody and hemoculture negative. The amplification of T. cruzi–specific DNA sequences in the presence of an elevated antibody titer and negative culture results suggests good sensitivity of this method for detecting the presence of the parasite in archival tissues.
机译：这项研究应用聚合酶链反应（PCR），使用3套克鲁斯锥虫特异引物从31个已归档福尔马林固定和新鲜冷冻的浣熊心脏中扩增DNA。 PCR成功地从福尔马林固定和新鲜冷冻的浣熊心脏心肌中多个部位成功扩增了克鲁斯特异性序列，该序列具有至少1个引物，先前已使用酶联免疫吸附测定和间接免疫荧光抗体滴度检测为阳性没有阳性血液培养结果。克鲁斯锥虫DNA最常从室间隔，右心室和左心房扩增。另外，在至少1只血培养呈阳性的浣熊和2只对克鲁斯氏抗体临界边界阴性且血液培养阴性的动物中，用所有3条引物扩增了克鲁维氏菌DNA。在抗体滴度升高和培养结果阴性的情况下，克鲁维弧菌特异性DNA序列的扩增表明该方法可检测档案组织中寄生虫的良好敏感性。

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