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首页> 外文期刊>Journal of Parasitology >IMMUNOELECTROTRANSFER BLOT ASSAY IN ACUTE AND CHRONIC HUMAN TRICHINELLOSIS
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IMMUNOELECTROTRANSFER BLOT ASSAY IN ACUTE AND CHRONIC HUMAN TRICHINELLOSIS

机译:急性和慢性人类三角虫病的免疫细胞印迹分析

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摘要

An immunoelectrotransfer blot assay (IETB) using excretory–secretory products of muscle larvae of Trichinella spiralis (ML-ESP) and the avidin–biotin system was developed in order to characterize reactivity against ML-ESP in sera from patients with acute and chronic trichinellosis. A complete pattern of up to 13 bands was developed by sera from individuals with trichinellosis where doublets, triplets, or single bands were shown to have molecular weights of roughly 66, 55, 45, 36, 29, 24, and 14 kDa. The bands at 55, 36, 29, and 14 kDa proved specific for T. spiralis. The band at 55 kDa was present in all trichinellosis sera, whereas the 14-kDa band was present in only a small percentage of sera. The development of 36- and 29-kDa bands suggests a modulation of the reactivity against ML-ESP over time. IETB proved more sensitive for the population of chronic trichinellosis under study than a conventional diagnostic enzyme-linked immunosorbent assay, allowing negative or borderline serum samples to be determined. Thus, this technique, when applied for human trichinellosis surveillance, should provide a useful tool in endemic areas.
机译:为了表征急性和慢性旋毛虫病患者血清中针对ML-ESP的反应性,开发了一种使用旋毛虫旋毛虫幼虫的排泄分泌产物(ML-ESP)和抗生物素蛋白-生物素系统的免疫电转移印迹分析(IETB)。血清由旋毛虫病的个体开发出多达13条带的完整模式,其中双峰,三胞胎或单条带显示分子量约为66、55、45、36、29、24和14 kDa。 55、36、29和14 kDa的条带被证明对螺旋藻具有特异性。在所有旋毛虫病血清中均存在55 kDa的条带,而仅一小部分血清中存在14 kDa的条带。 36 kDa和29 kDa条带的发展表明随着时间的流逝,针对ML-ESP的反应性有所调节。事实证明,IETB对正在研究的慢性旋毛虫病种群比常规诊断酶联免疫吸附测定法更为敏感,从而可以测定阴性或临界血清样品。因此,该技术用于人体旋毛虫病监测时,应在流行地区提供有用的工具。

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