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首页> 外文期刊>Journal of Plant Biology >Tiller formation in rice is altered by overexpression of OsIAGLU gene encoding an IAA-conjugating enzyme or exogenous treatment of free IAA
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Tiller formation in rice is altered by overexpression of OsIAGLU gene encoding an IAA-conjugating enzyme or exogenous treatment of free IAA

机译：水稻中分iller的形成因编码IAA结合酶的OsIAGLU基因的过表达或游离IAA的外源处理而改变

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摘要

Optimization of plant architecture is important for cultivation and yield of cereal crops in the field. Tillering is an essential factor used to determine the overall architecture of cereal crops. It has long been recognized that the development of branching patterns is controlled by the level and distribution of auxin within a plant. To better understand the relationship between auxin levels and tillering in rice, we examined rice plants with increased or decreased levels of free IAA. To decrease IAA levels, we selected the rice IAA-glucose synthase gene (OsIAGLU) from the rice genome database based on high sequence homology with IAA-glucose synthase from maize (ZmIAGLU), which is known to generate IAAglucose conjugate from free IAA. The OsIAGLU gene driven by the Cauliflower Mosaic Virus 35S promoter was transformed into a rice cultivar to generate transgenic rice plants constitutively over-expressing this gene. The number of tillers and panicles significantly increased in the transgenic lines compared to the wild-type plants, while plant height and panicle length decreased. These results indicate that decreased levels of free IAA likely enhance tiller formation in rice. To increase levels of free IAA, we treated rice plants with three different concentrations of exogenous IAA (1 μM, 10 μM and 100 μM) twice a week by spraying. Exogenous IAA treatment at concentrations of 10 μM and 100 μM significantly reduced tiller number in three different rice cultivars. These results indicate that exogenously applied IAA inhibits shoot branching in rice. Overall, auxin tightly controls tiller formation in rice in a negative way.
机译：优化植物结构对于田间谷物作物的种植和产量至关重要。分iller是用于确定谷物作物总体结构的重要因素。早已认识到，分支模式的发展受植物中生长素水平和分布的控制。为了更好地了解水稻中生长素水平与分till之间的关系，我们研究了游离IAA含量升高或降低的水稻植株。为了降低IAA水平，我们基于与玉米IAA-葡萄糖合酶（ZmIAGLU）的高序列同源性，从水稻基因组数据库中选择了水稻IAA-葡萄糖合酶基因（OsIAGLU），已知该IIA-葡萄糖合酶可以从游离IAA生成IAA葡萄糖共轭物。由花椰菜花叶病毒35S启动子驱动的OsIAGLU基因被转化为水稻品种，以产生组成型过量表达该基因的转基因水稻植物。与野生型植物相比，转基因品系中的分ers和穗数显着增加，而株高和穗长下降。这些结果表明，降低的游离IAA水平可能会促进水稻分till形成。为了增加游离IAA的水平，我们每周两次用三种不同浓度的外源IAA（1μM，10μM和100μM）处理水稻植株。在三种不同的水稻品种中，分别以10μM和100μM的浓度进行外源IAA处理可显着减少分till数。这些结果表明，外源施用的IAA抑制水稻的枝条分支。总体而言，生长素以负面的方式严格控制水稻分till的形成。

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  • 来源
    《Journal of Plant Biology》 |2012年第6期|p.429-435|共7页
  • 作者

    Min-Seon Choi; Eun-Byeol Koh; Mi-Ok Woo; Rihua Piao; Chang-Sik Oh; Hee-Jong Koh;

  • 作者单位

    Department of Plant Science, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Korea;

    Department of Plant Science, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Korea;

    Soybean Genomics and Improvement Laboratory, USDA, ARS, Beltsville, Maryland, 20705, USA;

    Department of Plant Science, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Korea;

    Department of Horticultural Biotechnology and Institute of Life Sciences &amp Resources, Kyung Hee University, Yong-In, 446-701, SKorea;

    Department of Plant Science, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National Un;

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  • 正文语种 eng
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  • 关键词

    Auxin-conjugate; IAA-glucose synthase; Rice tiller; Shoot branching; Transgenic plant;

    机译：生长素结合物;IAA-葡萄糖合酶;水稻分er;枝条;转基因植物;

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