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首页> 外文期刊>Journal of Polymers and the Environment >Synthesis of Short-chain-length/Medium-chain-length Polyhydroxyalkanoate (PHA) Copolymers in Peroxisome of the Transgenic Arabidopsis Thaliana Harboring the PHA Synthase Gene from Pseudomonas sp. 61-3
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Synthesis of Short-chain-length/Medium-chain-length Polyhydroxyalkanoate (PHA) Copolymers in Peroxisome of the Transgenic Arabidopsis Thaliana Harboring the PHA Synthase Gene from Pseudomonas sp. 61-3

机译：短链/中链聚羟基链烷酸酯（PHA）共聚物在拟南芥中过氧化物酶体中的合成，该过氧化物酶体携带假单胞菌属物种的PHA合酶基因。 61-3

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In this paper, the photosynthetic production of short-chain-length/medium-chain-length polyhy-droxyalkanoate (PHA) copolymers is reported. The wild-type and highly active doubly mutated PHA synthase 1 (S325T/Q481K, abbreviated ST/QK) genes from Pseudomonas sp. 61-3 were introduced into Arabidopsis thaliana. Peroxisome targeting signal 1 (PTS1) was used to target PHA synthases into the peroxisome to synthesize PHA from the intermediates of the β-oxidation pathway. The transgenic Arabidopsis produced PHA copolymers consisting of 40-57 mol% 3-hydroxybutyrate, 21-49 mol% 3-hydroxy-valerate, 8-18 mol% 3-hydroxyhexanoate, and 2-8 mol% 3-hydroxyoctanoate. The maximum PHA contents were 220μ g/g cell dry weight (cdw) in leaves, and 36μ g/g cdw in stems, respectively. The expression of the ST/QK mutated PHA synthase in leaves gene did not lead to significant difference in PHA content and monomer composition of PHAs, compared to the wild-type PHA synthase gene, suggesting that the supply of monomers may be a rate-determining step of PHA biosynthesis in the peroxisome. However, in stems, there were significant differences dependent on whether the wild-type or ST/QK mutated PHA synthase was expressed. These results suggest that tissue-specific monomer availability is important in determining the final mol% composition of PHA copolymers produced by the peroxisome in plants.
机译：本文报道了短链/中链长度的聚羟基链烷酸酯（PHA）共聚物的光合作用。来自假单胞菌属（Pseudomonas sp。）的野生型和高活性双突变的PHA合酶1（S325T / Q481K，缩写为ST / QK）基因。将61-3引入到拟南芥中。过氧化物酶体靶向信号1（PTS1）用于将PHA合成酶靶向到过氧化物酶体中，以从β-氧化途径的中间体合成PHA。转基因拟南芥生产的PHA共聚物由40-57mol％的3-羟基丁酸酯，21-49mol％的3-羟基-戊酸酯，8-18mol％的3-羟基己酸酯和2-8mol％的3-羟基辛酸酯组成。叶片中最大的PHA含量为220μg / g细胞干重（cdw），茎中的最大PHA含量为36μg / g CDw。与野生型PHA合酶基因相比，叶片基因中ST / QK突变的PHA合酶的表达并未导致PHA含量和PHAs单体组成的显着差异，这表明单体的供应可能是决定速率的因素过氧化物酶体中PHA生物合成的步骤。但是，在茎中，取决于是否表达野生型或ST / QK突变的PHA合酶，存在显着差异。这些结果表明，组织特异性单体的可用性对于确定过氧化物酶体在植物中产生的PHA共聚物的最终摩尔％组成很重要。

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