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首页> 外文期刊>Journal of Polymers and the Environment >Production of Polyhydroxybutyrate from Ralstonia eutropha H-16 Using Makgeolli Lees Enzymatic Hydrolysate
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Production of Polyhydroxybutyrate from Ralstonia eutropha H-16 Using Makgeolli Lees Enzymatic Hydrolysate

机译：用麦格利利酒糟酶解产物从富营养小球藻H-16生产多羟基丁酸酯

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摘要

The objective of this study was to investigate the potential use of waste Makgeolli lees (ML), a by-product of Makgeolli production, as a feedstock for biorefinery production of polyhydroxybutyrate (PHB). ML enzymatic hydrolysate (MLEH) was prepared from ML subjected to liquefaction with amylase and subsequent saccharification with glucoamylase. Since MLEH contains glucose (81g/L) and protein (39g/L) among other elements, it was used as the carbon source for PHB production by Ralstonia eutropha H-16. After flask incubation with MLEH, the obtained biomass and PHB content were 10.03g/L and 34%, respectively, which were higher than the contents obtained when using glucose as the sole carbon source. However, batch fermentation resulted in low PHB accumulation despite suitable cell growth being observed, and these results were thought to be due to the unbalanced C/N ratio. To control the C/N ratio to 20:1, sterile glucose solution was added twice in the fed-batch fermentation with MLEH, and we could obtained dry cell weight and PHB of 36.9g/L and 24.1g/L, respectively. Therefore, this study demonstrated the potential use of waste ML as a feedstock for industrial PHB production, which has the important advantage of reduced production cost.
机译：这项研究的目的是研究将Makgeolli生产的副产品Makgeolli酒糟（ML）废料作为生物精炼生产聚羟基丁酸酯（PHB）的原料的潜在用途。 ML酶解产物（MLEH）是由ML制备的，该淀粉经淀粉酶液化并随后用葡糖淀粉酶糖化。由于MLEH除其他元素外还包含葡萄糖（81g / L）和蛋白质（39g / L），因此已被用作富营养小al R-16的碳源。烧瓶与MLEH孵育后，所得生物量和PHB含量分别为10.03g / L和34％，高于使用葡萄糖作为唯一碳源时的含量。然而，尽管观察到适当的细胞生长，但分批发酵导致低PHB积累，这些结果被认为是由于C / N比不平衡所致。为了将C / N比控制为20：1，在MLEH分批补料发酵中添加了无菌葡萄糖溶液两次，得到的干细胞重量和PHB分别为36.9g / L和24.1g / L。因此，本研究证明了将废ML用作工业PHB生产的原料的潜在用途，这具有降低生产成本的重要优势。

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