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首页> 外文期刊>Journal of Polymers and the Environment >Production of Polyhydroxybutyrate from Ralstonia eutropha H-16 Using Makgeolli Lees Enzymatic Hydrolysate
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Production of Polyhydroxybutyrate from Ralstonia eutropha H-16 Using Makgeolli Lees Enzymatic Hydrolysate

机译：使用Makgeolli Lees酶水解产物从Ralstonia Eutropha H-16产生多羟基丁酸酯

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摘要

The objective of this study was to investigate the potential use of waste Makgeolli lees (ML), a by-product of Makgeolli production, as a feedstock for biorefinery production of polyhydroxybutyrate (PHB). ML enzymatic hydrolysate (MLEH) was prepared from ML subjected to liquefaction with amylase and subsequent saccharification with glucoamylase. Since MLEH contains glucose (81g/L) and protein (39g/L) among other elements, it was used as the carbon source for PHB production by Ralstonia eutropha H-16. After flask incubation with MLEH, the obtained biomass and PHB content were 10.03g/L and 34%, respectively, which were higher than the contents obtained when using glucose as the sole carbon source. However, batch fermentation resulted in low PHB accumulation despite suitable cell growth being observed, and these results were thought to be due to the unbalanced C/N ratio. To control the C/N ratio to 20:1, sterile glucose solution was added twice in the fed-batch fermentation with MLEH, and we could obtained dry cell weight and PHB of 36.9g/L and 24.1g/L, respectively. Therefore, this study demonstrated the potential use of waste ML as a feedstock for industrial PHB production, which has the important advantage of reduced production cost.
机译：本研究的目的是调查潜在使用废像Gakeolli Lees（ml），Makgeolli生产的副产物，作为用于聚羟丁酸酯（PHB）的生物遗产生产的原料。用ML与氨基酶和随后用葡糖淀粉酶进行糖化酶制备Ml酶水解产物（MLEH）。由于MLEH含有葡萄糖（81g / L）和蛋白质（39g / L），因此使用Ralstonia Eutropha H-16作为PHB产生的碳源。在烧瓶孵育后，所得生物质和PHB含量分别为10.03g / L和34％，其高于使用葡萄糖作为唯一碳源时获得的内容物。然而，尽管观察到适当的细胞生长，分批发酵导致低pHB积累，并且认为这些结果是由于C / N比不平衡。为了控制C / N比至20：1，用MLEH的FED批量发酵中加入两次无菌葡萄糖溶液，我们可以分别获得干细胞重量和36.9g / L和24.1g / L的pHB。因此，本研究证明了废物ML作为工业PHB生产原料的潜在用途，这具有降低生产成本的重要优势。

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