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首页> 外文期刊>Journal of Polymers and the Environment >Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock
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Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock

机译：利用廉价碳原料改善芽孢杆菌内霉素的多羟基烷酸盐的生产和表征

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摘要

The study aimed at the production and characterization of biopolymer polyhydroxyalkanoate (PHA) by Bacillus endophyticus. The usage of refined (sucrose 2-4%) and inexpensive unrefined (sugar cane molasses 2-4%) carbon source was evaluated by varying the media components via shake flask method and batch cultivation in bioreactor. The optimal PHA production of 10.7g/L of PHA from 15.37g/L of cell density was obtained using 4% sugarcane molasses (SCM) at 250rpm using optimized medium obtained through statistical experimental design. Batch cultivation with increased agitation rate and addition of feeding nutrients provided by SCM significantly lead to enhanced PHA production by the test organism by increasing the overall cell density. According to the statistical model, the increase in the sugar concentration (4%) by SCM had a negative effect on PHA production. Characterization by FTIR and H-1 NMR was performed to confirm the biopolymer that has been produced by Bacillus spp.
机译：研究旨在通过芽孢杆菌生物聚合物多羟基烷酸酯（PHA）的生产和表征。通过摇瓶方法和生物反应器分批培养来评估精制（蔗糖2-4％）和廉价未定义（甘蔗糖蜜2-4％）碳源的用途。使用通过统计实验设计获得的优化培养基，在250rpm下在250rpm下使用4％的甘蔗糖蜜（SCM）获得10.7g / L的PHA的最佳PHA产生。分批培养具有增加的搅拌速率，并通过SCM提供的饲养营养物质通过增加整体细胞密度来提高测试生物的PHA产生。根据统计模型，SCM的糖浓度（> 4％）的增加对PHA生产具有负面影响。进行FTIR和H-1 NMR的表征以确认已由Bacillus SPP生产的生物聚合物。

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