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首页> 外文期刊>Journal of Polymers and the Environment >Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock
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Improvement of the Production and Characterisation of Polyhydroxyalkanoate by Bacillus endophyticus Using Inexpensive Carbon Feedstock

机译：利用廉价的碳原料改善内生芽孢杆菌对多羟基链烷酸酯的生产和表征

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摘要

The study aimed at the production and characterization of biopolymer polyhydroxyalkanoate (PHA) by Bacillus endophyticus. The usage of refined (sucrose 2-4%) and inexpensive unrefined (sugar cane molasses 2-4%) carbon source was evaluated by varying the media components via shake flask method and batch cultivation in bioreactor. The optimal PHA production of 10.7g/L of PHA from 15.37g/L of cell density was obtained using 4% sugarcane molasses (SCM) at 250rpm using optimized medium obtained through statistical experimental design. Batch cultivation with increased agitation rate and addition of feeding nutrients provided by SCM significantly lead to enhanced PHA production by the test organism by increasing the overall cell density. According to the statistical model, the increase in the sugar concentration (4%) by SCM had a negative effect on PHA production. Characterization by FTIR and H-1 NMR was performed to confirm the biopolymer that has been produced by Bacillus spp.
机译：该研究旨在通过内生芽孢杆菌生产和表征生物聚合物聚羟基链烷酸酯（PHA）。通过摇瓶法改变培养基成分并在生物反应器中分批培养，评估了精制（蔗糖2-4％）和便宜的未精制（甘蔗糖蜜2-4％）碳源的使用。使用4％甘蔗糖蜜（SCM）在250rpm下使用通过统计实验设计获得的优化培养基，从15.37g / L细胞密度中获得了10.7g / L PHA的最佳PHA产量。通过增加SCM提供的搅拌速度和添加营养来进行分批培养，可通过增加总体细胞密度显着提高受试生物的PHA产量。根据统计模型，SCM所增加的糖浓度（> 4％）会对PHA产生负面影响。通过FTIR和H-1 NMR进行表征以确认由芽孢杆菌属（Bacillus spp）产生的生物聚合物。

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