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首页> 外文期刊>Journal of Polymers and the Environment >Microbial Cellulose from a Komagataeibacter intermedius Strain Isolated from Commercial Wine Vinegar
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Microbial Cellulose from a Komagataeibacter intermedius Strain Isolated from Commercial Wine Vinegar

机译：来自komagataibacter中毒素菌株的微生物纤维素从商业葡萄酒醋中分离

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摘要

In this study a new bacterial cellulose (BC) producer isolated from commercial vinegar is identified as Komagataeibacter intermedius JF2 based on the examination of general taxonomical characteristics, 16S rDNA sequence analysis, and MALDI-TOF mass spectrometry. The cellulose produced is studied in terms of morphology by scanning electron microscopy, crystallinity by X-Ray diffraction, structure by Fourier transform infrared spectroscopy, and water absorption capacity. BC yield and characteristics of the cellulose produced by the new isolated JF2 are compared with those of the well-known and commonly-used BC producer Komagataeibacter xylinus. Yield of cellulose production was higher for JF2 than for K. xylinus grown on several culture media. JF2 exhibited maximum BC production (1.6g/L) growing on HS medium supplemented with mannitol. The molecular structure of the produced cellulose was the same for both strains and it was in concordance with that of BC. The nanocellulose fibers produced by JF2 showed a higher degree of crystallinity and a more homogeneous size distribution than those produced by K. xylinus. The results suggested that Komagataeibacter intermedius JF2 could be a suitable candidate as a BC producer for biotechnological applications.
机译：在本研究中，基于一般分类学特征的检查，16S rDNA序列分析和MALDI-TOF质谱法，将从商业醋分离的新的细菌纤维素（BC）生产者鉴定为Komagataibacter中间审友JF2。通过扫描电子显微镜，通过X射线衍射，结构通过傅里叶变换红外光谱和吸水能力，在形态学中研究产生的纤维素。通过新的孤立的JF2产生的纤维素的BC产量和特征与众所周知的和普通使用的BC生产商Komagataeibacter木质素的纤维素进行比较。对于在几种培养基上生长的K.Xylinus而言，JF2的纤维素产量较高。 JF2在补充甘露醇的HS培养基上表现出最大的BC生产（1.6g / L）。产生的纤维素的分子结构对于两个菌株相同，并且它与BC的一致性。通过JF 2制备的纳米纤维纤维显示出比K.木髓产生的结晶度较高的结晶度和更均匀的尺寸分布。结果表明，Komagataeibacter jf2可以是作为生物技术应用的BC生产者的合适候选者。

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  • 来源
    《Journal of Polymers and the Environment》 |2019年第5期|956-967|共12页
  • 作者

    Fernandez Julia; Gala Morena A.; Valenzuela Susana V.; Javier Pastor F. I.; Diaz Pilar; Martinez Josefina;

  • 作者单位

    Univ Barcelona Dept Genet Microbiol & Stat Fac Biol Av Diagonal 643 E-08028 Barcelona Spain|Univ Politecn Catalunya BarcelonaTech CELBIOTECH Paper Engn Res Grp Terrassa 08222 Spain;

    Univ Barcelona Dept Genet Microbiol & Stat Fac Biol Av Diagonal 643 E-08028 Barcelona Spain;

    Univ Barcelona Dept Genet Microbiol & Stat Fac Biol Av Diagonal 643 E-08028 Barcelona Spain|Univ Barcelona Inst Nanosci & Nanotechnol IN2UB Barcelona Spain;

    Univ Barcelona Dept Genet Microbiol & Stat Fac Biol Av Diagonal 643 E-08028 Barcelona Spain|Univ Barcelona Inst Nanosci & Nanotechnol IN2UB Barcelona Spain;

    Univ Barcelona Dept Genet Microbiol & Stat Fac Biol Av Diagonal 643 E-08028 Barcelona Spain|Univ Barcelona Inst Nanosci & Nanotechnol IN2UB Barcelona Spain;

    Univ Barcelona Dept Genet Microbiol & Stat Fac Biol Av Diagonal 643 E-08028 Barcelona Spain|Univ Barcelona Inst Nanosci & Nanotechnol IN2UB Barcelona Spain;

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  • 正文语种 eng
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  • 关键词

    Bacterial cellulose; Strain selection; Komagataeibacter intermedius; Mannitol; Pellicle physicochemical properties;

    机译：细菌纤维素;菌株选择;Komagataeibacter中间体;甘露醇;薄膜物理化学性质;

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