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首页> 外文期刊>Journal of Polymers and the Environment >Production of Poly(3-hydroxyalkanoate)s by Pseudomonas putida Cultivated in a Glycerol/Nonanoic Acid-Containing Medium
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Production of Poly(3-hydroxyalkanoate)s by Pseudomonas putida Cultivated in a Glycerol/Nonanoic Acid-Containing Medium

机译：在含有甘油/壬酸的培养基中培养恶臭假单胞菌生产聚3-羟基链烷酸酯

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摘要

The biosynthesis of poly(3-hydroxyalkanoate) (PHA) by Pseudomonas putida (JCM6160) cultivated in a medium containing glycerol, nonanoic acid, or a glycerolonanoic acid mixture as the sole carbon sources was investigated. The PHA content was ~20 % when glycerol was the carbon source. This relatively low content can be attributed to the glycerol end-cap effect and the absence of enzymes that can directly synthesize PHA from acetyl CoA, which is the major metabolite of glycerol. Fatty acids, containing even numbered carbons, are synthesized from acetyl CoA, and they can be used as substrates for PHA synthesis. However, this process also results in decreasing PHA content as fatty acids are siphoned off into other pathways. However, addition of 5 mM nonanoic acid into a 20 mM glycerol-containing medium dramatically increased the PHA content in P. putida, which was 1.3 times larger than the sum of the values found when glycerol and nonanoic acid were each used as the sole carbon source. The PHA, synthesized in the glycerolonanoic acid medium, contains 3-hydroxy alkanoate units that have 5, 6, 7, 8, 9, or 10 carbons. The units that contain the even numbered carbons are derived from fatty acids that were produced from glycerol; whereas, the PHA units with the odd numbered carbons are derived from nonanoic acid. Pentanoate units were also found in the polyester derived from glycerol and nonanoic acid, and must have been synthesized indirectly via β-oxidation of nonanoic acid with the assistance of glycerol because pentanoate units were not found in PHA when P. putida was cultivated in the presence of only nonanoic acid.
机译：研究了在含有甘油，壬酸或甘油/壬酸混合物作为唯一碳源的培养基中培养的恶臭假单胞菌（JCM6160）对聚（3-羟基链烷酸酯）（PHA）的生物合成。当甘油为碳源时，PHA含量约为20％。这种相对较低的含量可归因于甘油的封端效应和不存在可直接从乙酰CoA合成PHA的酶，乙酰CoA是甘油的主要代谢产物。含偶数碳的脂肪酸是从乙酰CoA合成的，可用作PHA合成的底物。但是，随着脂肪酸被虹吸进入其他途径，该过程还会导致PHA含量降低。但是，在20 mM的含甘油培养基中添加5 mM壬酸会显着增加恶臭假单胞菌中PHA的含量，这比甘油和壬酸分别用作唯一碳时发现的值之和大1.3倍资源。在甘油/壬酸介质中合成的PHA包含具有5、6、7、8、9或10个碳的3-羟基链烷酸酯单元。包含偶数碳的单元衍生自甘油产生的脂肪酸；然而，具有奇数碳原子的PHA单元衍生自壬酸。在衍生自甘油和壬酸的聚酯中也发现了戊酸酯单元，并且必须通过甘油辅助壬酸的β-氧化反应间接合成戊酸酯单元，因为在存在恶臭假单胞菌的情况下在PHA中未发现戊酸酯单元。仅壬酸。

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