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首页> 外文期刊>Journal of Polymers and the Environment >Anti-biofilm Activity of Solvent-Cast and Electrospun Polyhydroxyalkanoate Membranes Treated with Lysozyme
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Anti-biofilm Activity of Solvent-Cast and Electrospun Polyhydroxyalkanoate Membranes Treated with Lysozyme

机译：溶菌酶处理的溶剂型和电纺聚羟基链烷酸酯膜的抗生物膜活性

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摘要

Biofilms consist of groups of microorganisms that adhere to surfaces, such as wound and implant surfaces, making it difficult to prevent or remove their formation by antibiotic treatment, due to the innate resistance of the biofilm. Effective treatments of medical biofilms are limited. Polyhydroxyalkanoate (PHA) is a biodegradable and biocompatible polymer that is a suitable alternative to petroleum based polymers for use as a raw material for medical applications. In this study, membranes of the copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] containing different HHx monomer contents were used due to its porosity and flexibility, and different sheets were prepared by solvent-casting and electrospinning methods. The sheets were loaded with lysozyme in order to measure the maximum amount of protein adsorption and to examine the ability of immobilized enzyme to inhibit biofilm formation and detach previously established biofilms. Our results have shown maximum loading of 16.1 A mu g enzyme per 9.5 mm(3) discs, and these sheets are effective for inhibiting biofilm formation. Also, lysozyme loaded, eletrospun sheets were observed to more effectively inhibit biofilm formation, as compared to solvent-cast sheets. Based on this study, P(HB-co-HHx) sheets are a suitable material for being used as a potential raw material for fabrication of wound dressings to be used in anti-biofilm treatments.
机译：生物膜由粘附在表面（如伤口和植入物表面）上的微生物组成，由于生物膜的先天抗性，很难通过抗生素处理防止或去除其形成。医用生物膜的有效治疗受到限制。聚羟基链烷酸酯（PHA）是一种可生物降解的生物相容性聚合物，是石油基聚合物的合适替代品，可用作医疗应用的原料。在这项研究中，由于孔隙率和柔韧性的原因，使用了含有不同HHx单体含量的共聚物聚（3-羟基丁酸酯-co-3-羟基己酸酯）[P（HB-co-HHx）]膜，并通过以下方法制备了不同的片材：溶剂浇铸和静电纺丝方法。薄片上装有溶菌酶，以测量最大的蛋白质吸附量，并检查固定化酶抑制生物膜形成和分离先前建立的生物膜的能力。我们的结果表明，每9.5毫米（3）光盘的最大负载量为16.1微克酶，这些纸片可有效抑制生物膜的形成。而且，与溶剂浇铸薄板相比，观察到溶菌酶负载的静电纺丝薄板更有效地抑制生物膜的形成。根据这项研究，P（HB-co-HHx）片是一种合适的材料，可用作制造抗生物膜治疗用伤口敷料的潜在原材料。

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