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首页> 外文期刊>Journal of Polymers and the Environment >Investigation of Biosurfactant Activity and Asphaltene Biodegradation by Bacillus cereus
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Investigation of Biosurfactant Activity and Asphaltene Biodegradation by Bacillus cereus

机译：蜡状芽孢杆菌的生物表面活性剂活性和沥青质的生物降解研究

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In this research, a biosurfactant-producing bacterium with capability of asphaltene degradation was isolated from oil-contaminated soil samples, and identified as Bacillus cereus. This strain produced an effective biosurfactant in the presence of molasses and the surface tension was reduced to the level of 36.4 mN/m after 48 h under optimum conditions. The optimum values of carbon-to-nitrogen ratio (C:N), pH, and temperature for biosurfactant production were determined as 30:1, 7.3 and 29 A degrees C, respectively, using response surface methodology. The maximum emulsification activity in the culture broth was 53 % after 48 h using kerosene at 25 A degrees C. The goodness of fit of four growth kinetic models including Tessier, Contois, Logistic and Westerhoff was compared for the bacterial growth and molasses utilization of B. cereus in 5-L batch bioreactor during 120 h. Conducted kinetic study showed that biosurfactant production had a good fit with the Contois growth kinetic model (R-2 = 0.962) and the maximum specific growth rate (A mu (max) ), saturation constant (K (s) ) and the yield of biomass per substrate (Y (x/s) ) were determined to be 0.145 h(-1), 1.83 g/L and 0.428 g/g, respectively. The asphaltene biodegradation in flask was evaluated by FTIR analysis and quantified by a spectrophotometer. This bacterium was able to degrade up to 40 % of asphaltene as a sole carbon and energy source after 60 days at 28 A degrees C. The resulting surface tension of 30.2 mN/m with the critical micelle concentration of 23.4 mg/L indicated good efficiency of the biosurfactant.
机译：在这项研究中，从石油污染的土壤样品中分离出具有沥青质降解能力的生物表面活性剂生产细菌，并将其鉴定为蜡状芽孢杆菌。该菌株在存在糖蜜的情况下产生了有效的生物表面活性剂，在最佳条件下48 h后，表面张力降低至36.4 mN / m。使用响应表面方法，确定生物表面活性剂生产的碳氮比（C：N），pH和温度的最佳值分别为30：1、7.3和29 A摄氏度。在25 A的温度下使用煤油在48 h后，培养液中的最大乳化活性为53％。比较了包括Tessier，Contois，Logistic和Westerhoff在内的四种生长动力学模型对B细菌生长和糖蜜利用的拟合优度。在120小时内在5-L分批生物反应器中进行蜡状芽孢杆菌进行的动力学研究表明，生物表面活性剂的生产与Contois生长动力学模型（R-2 = 0.962）以及最大比生长速率（A mu（max）），饱和常数（K（s））和确定每个底物的生物量（Y（x / s））为0.145 h（-1），1.83 g / L和0.428 g / g。通过FTIR分析评估烧瓶中的沥青质生物降解并通过分光光度计定量。该细菌在28 A的温度下放置60天后，能够降解高达40％的沥青质作为唯一的碳和能源。所产生的表面张力为30.2 mN / m，临界胶束浓度为23.4 mg / L，显示出良好的效率生物表面活性剂。

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