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首页> 外文期刊>Journal of Polymers and the Environment >Gene Cloning and Characterization of a Poly(L-Lactic Acid) Depolymerase from Pseudomonas sp. Strain DS04-T
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Gene Cloning and Characterization of a Poly(L-Lactic Acid) Depolymerase from Pseudomonas sp. Strain DS04-T

机译：假单胞菌属（Pseudomonas sp。）的聚（L-乳酸）解聚酶的基因克隆和表征。应变DS04-T

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摘要

A gene encoding a poly(L-lactic acid) (PLA) depolymerase from Pseudomonas sp. strain DS04-T was cloned and overexpressed in Escherichia coli. The recombi nant PLA depolymerase with a molecular weight of 19.2 kDa was purified to homogeneity. The optimum pH and temper ature of the PLA depolymerase are 8.5 and 60 °C, respec tively. K～+, Ca～2+ and Ni～2+ enhance the enzyme activity, while Na～+, Zn～2+, Mg～2+, Cu～2+, Fe～2+, Mn～2+ and Co～2+ inhibit it. The inhibition of different chemicals on the PLA depolymerase activity were examined, in which EDTA was found to have a significantly inhibitory effect. The main degradation product of the depolymerase is identified as lactic acid monomer by mass spectrometric analysis. Physicochemical properties, substrate specificity and sequence analysis indicated that PME is a new type of PLA depolymerase.
机译：编码假单胞菌属（Pseudomonas sp。）的聚（L-乳酸）（PLA）解聚酶的基因。菌株DS04-T被克隆并在大肠杆菌中过表达。将分子量为19.2 kDa的重组PLA解聚酶纯化至均质。 PLA解聚酶的最佳pH和温度分别为8.5和60°C。 K〜+，Ca〜2 +和Ni〜2 +增强了酶的活性，而Na〜+，Zn〜2 +，Mg〜2 +，Cu〜2 +，Fe〜2 +，Mn〜2 +和Co〜 2+抑制它。检查了不同化学物质对PLA解聚酶活性的抑制作用，其中发现EDTA具有明显的抑制作用。解聚酶的主要降解产物通过质谱分析鉴定为乳酸单体。理化性质，底物特异性和序列分析表明PME是一种新型的PLA解聚酶。

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  • 来源
    《Journal of Polymers and the Environment》 |2011年第4期|p.827-833|共7页
  • 作者

    Zhan-Yong Wang; Fan Li; Zi-Qi Guo; Yan Wang; Shan Chen;

  • 作者单位

    School of Life Sciences, Northeast Normal University,Changchun 130024, People's Republic of China,School of Environmental and Biological Engineering, Liaoning Shihua University, Fushun 113001, People's Republic of China;

    School of Life Sciences, Northeast Normal University,Changchun 130024, People's Republic of China;

    School of Life Sciences, Northeast Normal University,Changchun 130024, People's Republic of China;

    School of Life Sciences, Northeast Normal University,Changchun 130024, People's Republic of China;

    School of Life Sciences, Northeast Normal University,Changchun 130024, People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    poly(l-lactic acid); depolymerase; pseudomonas sp. Strain Ds04-T; recombinant;

    机译：聚（l-乳酸）;解聚酶假单胞菌菌株Ds04-T;重组的;

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