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FEASIBILITY OF USING WASTE MATERIALS AS FEEDSTOCKS FOR ETHANOL PRODUCTION

机译:将废料用作生产乙醇的原料的可行性

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For the purpose of exploring low-cost feedstocks for ethanol production and seeking environmental-friendly alternatives in waste disposal, enzymatic hydrolysis of cellulose from three types of feedstock from waste streams were studied: crop residues, poultry manure, and sewage sludge. The crop residues investigated include corn stalk and bagasse; they were pre-treated with KOH prior to enzymatic hydrolysis. At 40℃, with an enzyme loading of 800 units/g substrate, the glucose yields from enzymatic hydrolysis of corn stalk and bagasse were 65.4 ± 2.3% and 51.1 ± 2.3%, respectively. The enzymatic hydrolysis of corn stalk was further optimized by using wet substrate, which was grinded before KOH treatment, and by adding half of the enzyme dosage at the beginning of the process and half in the middle of the hydrolysis instead of adding the entire dosage at the beginning. For poultry manure, the highest glucose conversion was achieved when it was treated with KOH and then dried and grinded prior to enzymatic hydrolysis. At operating conditions of 40℃ and enzyme loading 400 units/g substrate, the glucose yield from poultry manure was 27.6±1.2%. Under the same optimum conditions as those observed for crop residues, 31.1±2.7% of wet primary sludge was converted to glucose, and it was increased to 54.2±4.0% when HCl and KOH pre-treatments were employed.
机译:为了探索用于乙醇生产的低成本原料并在废物处置中寻求环保替代品,研究了从废物流中的三种原料中酶水解纤维素的方法:作物残渣,家禽粪便和污水污泥。调查的农作物残留物包括玉米秸秆和蔗渣;在酶促水解之前,先用KOH对其进行预处理。在40℃,底物酶负荷为800单位/ g的条件下,玉米秸秆和甘蔗渣酶水解得到的葡萄糖产率分别为65.4±2.3%和51.1±2.3%。玉米秸秆的酶促水解进一步优化,方法是使用湿底物(在KOH处理之前将其研磨),并在过程开始时添加一半的酶剂量,在水解过程中添加一半的酶,而不是在水解过程中添加全部剂量。开始。对于家禽粪便,当用KOH处理然后在酶促水解之前进行干燥和研磨时,可获得最高的葡萄糖转化率。在40℃的操作条件下,酶负载量为400单位/ g底物时,家禽粪便的葡萄糖产率为27.6±1.2%。在与观察到的农作物残渣相同的最佳条件下,将31.1±2.7%的湿初级污泥转化为葡萄糖,当使用HCl和KOH预处理时,其增加至54.2±4.0%。

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