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首页> 外文期刊>The Journal of the Textile Institute >Effect of additives on mesophilic α-amylase and its application in the desizing of cotton fabrics
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Effect of additives on mesophilic α-amylase and its application in the desizing of cotton fabrics

机译:添加剂对嗜温α-淀粉酶的影响及其在棉织物退浆中的应用

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摘要

An investigation was carried out on thermal stability of -amylase. The influence of various additives (calcium acetate, sodium lactate, L-histidine, and water-soluble chitosan) on the stability of -amylase was studied. Results showed the inactivation behavior of -amylase with or without additives all followed the first-order kinetics. All additives (Ca2+, sodium lactate, L-histidine, and water-soluble chitosan) displayed good stabilizing effect on -amylase lower than 80 degrees C, and only water-soluble chitosan had an efficient stabilizing effect on -amylase when the treatment temperature exceeds 80 degrees C. All additives improved the catalytic activity of -amylase at 70-90 degrees C, and the appearance of water-soluble chitosan increased the catalytic activity of -amylase at 90 degrees C sharply. A desizing ratio of 68.42% was obtained by treating the cotton fabrics in the buffer solution at 100 degrees C without -amylase. To obtain a desizing ratio exceed 95% when fabrics were treated at 100 degrees C for 10min, the addition of water-soluble chitosan saves 2/3 -amylase dosage. Moreover, water-soluble chitosan showed a further improvement in desizing effect than the additive of calcium acetate.
机译:对淀粉酶的热稳定性进行了研究。研究了各种添加剂(乙酸钙,乳酸钠,L-组氨酸和水溶性壳聚糖)对淀粉酶稳定性的影响。结果表明,有或没有添加剂的-淀粉酶的失活行为都遵循一级动力学。所有添加剂(Ca2 +,乳酸钠,L-组氨酸和水溶性壳聚糖)对低于80摄氏度的淀粉酶都表现出良好的稳定作用,只有当处理温度超过80℃时,水溶性壳聚糖才对淀粉酶具有有效的稳定作用。 80°C。所有添加剂均提高了70-90°C时-淀粉酶的催化活性,水溶性壳聚糖的出现大大提高了90°C时-淀粉酶的催化活性。通过在缓冲液中在100℃下不使用淀粉酶处理棉织物而获得68.42%的退浆率。当织物在100摄氏度下处理10分钟时,要获得超过95%的退浆率,添加水溶性壳聚糖可节省2 / 3-淀粉酶的用量。此外,水溶性壳聚糖在退浆效果方面比乙酸钙添加剂进一步提高。

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