• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>繊維学会誌 >Successive Saccharification and Fermentation of Cellulosic Agricultural Residues Using a Combination of Cellulase and Recombinant Yeast
【24h】

Successive Saccharification and Fermentation of Cellulosic Agricultural Residues Using a Combination of Cellulase and Recombinant Yeast

机译：纤维素酶和重组酵母相结合的纤维素农残的连续糖化和发酵

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bioethanol was produced from agricultural residues in Hokkaido Prefecture, Japan, by dilute alkali pretreatment, and then successive saccharification with cellulase and fermentation with recombinant yeast pYBGA1 that is a recombinant laboratory yeast expressing β-glucosidase. The agricultural residues, beet pulp, beet leaf, corn stover, and weed were pretreated with 2% NaOH aqueous solution for 1 h at 121℃ to remove lignin. The pretreated beet pulp (20 g/L), for example, was then saccharified for 24 h by Cellic CTec (10 wt% to beet pulp) as the cellulase at 50℃ to give glucose (11.8 g/L) at relatively good rates' After the temperature decreased to 30℃, the resulting glucose was continuously fermented for 36 h by addition of 1 × 10~8 cells/mL of pYBGA1 yeast to give 5.54 g/L of bioethanol in 60% and 92% theoretical yields of cellulose in beet pulp and glucose saccharified, respectively. Other agricultural residues also afforded bioethanol by the successive saccharification and fermentation. It was found that the appropriate cellulase for saccharification differed with the kind of agricultural residue ； beet pulp was effectively saccharified by Cellic CTec, beet leaf and corn stover by Sucrose C， and weed by Sumizyme C. These agricultural residues were found to be suitable for the production of bioethanol because Hokkaido Prefecture is the most abundant producer of beets and corn in Japan, and accordingly, agricultural residues are easily available. pYBGA1 was also found to be a good yeast for the fermentation of sugars saccharified from agricultural residues.
机译：生物乙醇由日本北海道地区的农业残留物经稀碱预处理制成，然后连续进行纤维素酶糖化和重组酵母pYBGA1发酵，重组酵母pYBGA1是表达β-葡萄糖苷酶的重组实验室酵母。将农业残留物，甜菜浆，甜菜叶，玉米秸秆和杂草在2℃NaOH水溶液中于121℃预处理1小时，以去除木质素。例如，将经过预处理的甜菜浆（20 g / L）在50℃下用Cellic CTec（甜菜浆的10 wt％）作为纤维素酶糖化24小时，以相对较高的速率得到葡萄糖（11.8 g / L）在温度降至30℃后，通过添加1×10〜8细胞/ mL的pYBGA1酵母，连续发酵葡萄糖36小时，以60％和92％的理论纤维素产率得到5.54 g / L的生物乙醇。在甜菜浆和葡萄糖中分别糖化。通过连续的糖化和发酵，其他农业残留物也提供了生物乙醇。发现适合于糖化的纤维素酶因农业残留物的种类而异；甜菜浆被Cellic CTec有效糖化，蔗糖C被甜菜叶和玉米秸秆有效糖化，而Sumizyme C被杂草有效糖化。因为北海道县是日本甜菜和玉米最丰富的生产国，因此可以生产生物乙醇，因此农业残留物很容易获得。还发现pYBGA1是用于发酵从农业残留物中糖化的糖的良好酵母。

著录项

  • 来源
    《繊維学会誌》 |2014年第9期|191-196|共6页
  • 作者

    Xuzhe Zhao; Xianxiang Liang; Shuqin Han; Toshiyuki Uryu; Takashi Yoshida;

  • 作者单位

    Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Hokkaido, Japan;

    Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Hokkaido, Japan;

    Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Hokkaido, Japan;

    Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Hokkaido, Japan;

    Department of Bio and Environmental Chemistry, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Hokkaido, Japan,Research Center for Environmentally Friendly Materials Engineering, Muroran Institute of Technology, 27-1 Mizumoto-cho, Muroran 050-8585, Hokkaido, Japan;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Agricultural residue; Hokkaido Prefecture; Cellulases; pYBGA1 yeast; Successive saccharification and fermentation; Bioethanol;

    机译：农业残留物;北海道纤维素酶;pYBGA1酵母;连续糖化和发酵;生物乙醇;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号