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首页> 外文期刊>Journal of Vacuum Science & Technology. B, Microelectronics and Nanometer Structures >Desorption/ionization mass spectrometry on array of silicon microtips
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Desorption/ionization mass spectrometry on array of silicon microtips

机译:硅微尖端阵列上的解吸/电离质谱

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摘要

The matrix-free laser desorption/ionization of the biosamples for time-of-flight mass spectrometry from an array of gated silicon tips has been presented. The gated silicon tips were fabricated similar to the well-known electron-field-emitters gated sources. In this method, called desorption/ionization on silicon tips array, an array of gated silicon sharp tips covered with a biosample and irradiated by a pulsed ultraviolet laser serves as the effective source of ions, replacing standard ionization platforms. Tests have confirmed that the effective laser desorption/ionization of the low-mass biosamples, such as dopamine and leucyl-glycyl-glycine tripeptide, may be obtained. It is possible that the method will play a very important role in post-genetic proteomic investigations.
机译:已经提出了从一系列门控硅尖端中进行飞行时间质谱分析的生物样品的无基质激光解吸/电离。门控硅尖端的制造类似于众所周知的电子场发射极门控源。在这种方法中,称为硅尖端阵列上的解吸/电离,这是一个覆盖有生物样品并被脉冲紫外激光照射的门控硅尖端阵列,作为离子的有效来源,代替了标准电离平台。测试证实,可以对低质量生物样品(例如多巴胺和亮氨酰-甘氨酰-甘氨酸三肽)进行有效的激光解吸/电离。该方法可能在后基因组蛋白质组学研究中发挥非常重要的作用。

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