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Automated standardization of images of Drosophila embryos

机译:果蝇胚胎图像的自动标准化

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Modeling expression patterns of Drosophila, in space and time, plays a critical role to understand the development of multicellular organisms. In confocal microscopy, to produce precise quantitative data it is frequently necessary to process and analyze large amounts of digital images. Automatic preprocessing is a crucial step in this scenario, essential to standardize significant features such as orientation, size, position, direction, lighting condition and texture of embryo images. Even though a lot of efforts have been made, a robust embryo standardization strategy is still needed. In this paper, we propose the method Embrystandar. It is designed to remove background artifacts and standardize the direction and orientation of a Drosophila embryo through a sequence of automatic operations. To test its potential for large-scale image processing, Embrystandar was applied in different databases. It showed to be robust and precise, reaching more than 90% success rate. (c) 2020 Elsevier Inc. All rights reserved.
机译:在空间和时间,果蝇的模拟表达模式起到了解多细胞生物的发展的关键作用。在共聚焦显微镜中,为了产生精确的定量数据,通常需要处理和分析大量数字图像。自动预处理是这种情况的重要步骤,对于标准化显着的特征,如方向,尺寸,位置,方向,照明条件和胚胎图像的纹理必不可少。尽管已经进行了很多努力,但仍然需要强大的嵌入标准化策略。在本文中,我们提出了纺织品的方法。它旨在通过一系列自动操作去除背景伪像并标准化果蝇胚胎的方向和方向。为了测试其大规模图像处理的潜力,嵌入式可应用于不同的数据库。它表明稳健和精确,达到90%以上的成功率。 (c)2020 Elsevier Inc.保留所有权利。

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