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首页> 外文期刊>Journal of Wood Chemistry and Technology >STRUCTURE ELUCIDATION AND EVALUATION OF ANTIOXIDANT AND TYROSINASE INHIBITORY EFFECT AND MECHANISM OF PROANTHOCYANIDINS FROM LEAF AND FRUIT OF LEUCAENA LEUCOCEPHAIA
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STRUCTURE ELUCIDATION AND EVALUATION OF ANTIOXIDANT AND TYROSINASE INHIBITORY EFFECT AND MECHANISM OF PROANTHOCYANIDINS FROM LEAF AND FRUIT OF LEUCAENA LEUCOCEPHAIA

机译：白花椰菜叶和果中抗花青素和酪氨酸酶抑制作用及原花青素作用机理的结构鉴定和评价

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摘要

This study aimed to investigate the structural features, antioxidant activity, tyrosinase inhibitory effect, and mechanism of proanthocyanidins from leaf and fruit of Leucaena leucocephala. MALDI-TOF-MS, thiolysis coupled with HPLC-ESI-MS, and ~(13)C-NMR confirmed that these proanthocyanidins were complex mixtures of propelargonidins, procyanidins, and prodelphinidins. (Epi)catechin, (epi)gallocatechin, (epi)catechin gallate, and (epi)gallocatechin gallate were the main constitutive units. The findings obtained from enzyme analysis revealed that the proanthocyanidins had inhibitory effects on both monophenolase and diphenolase of tyrosinase. The IC_(50) values of leaf and fruit proanthocyanidins were 73.5 ± 2.7 and 52.3 ± 3.5 μg/mL for monophenolase activity, and 27.2 ±1.4 and 16.1 ±1.1μg/mL for diphenolase activity, respectively. The inhibition of diphenolase by proanthocyanidins were proved to be reversible and mixed type. In addition, it was also found from various antioxidant methods that the proanthocyanidins in leaf and fruit possessed potent DPPH radical scavenging activity with IC_(50) values of 103.4 ± 0.8 and 87.8 ±1.1 μg/mL, ABTS radical scavenging activity with IC_(50) values of 72.9 ± 0.4 and 65.7 ± 0.9 μg/mL, and ferric reducing antioxidant power with FRAP values of 3.74 ±0.03 and 4.02 ± 0.15 mmol AAE/g, respectively. The results obtained suggested that the proanthocyanidins from leaf and fruit of L. leucocephala might have the potential to be exploited as natural tyrosinase inhibitors and antioxidants.
机译：本研究旨在探讨银合欢叶和果实中原花色素的结构特征，抗氧化活性，酪氨酸酶抑制作用及其机理。 MALDI-TOF-MS，硫解与HPLC-ESI-MS联用和〜（13）C-NMR证实，这些原花青素是丙炔诺丁素，原花青素和原卟啉的复杂混合物。主要的组成单元是表儿茶素，表儿茶素，表儿茶素没食子酸酯和表儿茶素没食子酸酯。从酶分析获得的发现表明原花青素对酪氨酸酶的单酚酶和双酚酶均具有抑制作用。叶和果原花色素的IC_（50）值对单酚酶活性分别为73.5±2.7和52.3±3.5μg/ mL，对二酚酶活性分别为27.2±1.4和16.1±1.1μg/ mL。原花青素对二酚酶的抑制作用是可逆的和混合型。此外，从各种抗氧化剂方法中还发现，叶和果实中的原花青素具有有效的DPPH自由基清除活性，IC_（50）值为103.4±0.8和87.8±1.1μg/ mL，ABTS自由基清除活性具有IC_（50 ）值分别为72.9±0.4和65.7±0.9μg/ mL，还原铁的抗氧化能力为FRAP，分别为3.74±0.03和4.02±0.15 mmol AAE / g。获得的结果表明，白头参叶和果中的原花色素可能具有被开发为天然酪氨酸酶抑制剂和抗氧化剂的潜力。

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