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首页> 外文期刊>Journal of Wood Chemistry and Technology >Enzymatic Hydrolysis of Carboxymethylcellulose and Filter Paper by Immobilized Cellulases on Lignophenols
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Enzymatic Hydrolysis of Carboxymethylcellulose and Filter Paper by Immobilized Cellulases on Lignophenols

机译：固定化纤维素酶在木质素酚上的酶水解羧甲基纤维素和滤纸

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摘要

The cost of cellulose is a major factor limiting the enzymatic hydrolysis of cellulosic biomass. Thus, immobilization of cellulose would be an important advancement. Lignophenol is a lignin-based functional phenolic polymer synthesized from a lignocellulosic material and a phenol derivative at ambient temperature. Cellulose derived from Trichoderma reesei is easily immobilized by softwood and hardwood lig-nocresol simply by mixing to produce a water-insoluble lignophenol-cellulase complex. Enzymatic hydrolysis performances of cellulases immobilized on lignocresols are approximately 80-90% and 30-50% relative to that of free cellulose in the hydrolysis of carboxymethylcellulose (CMC) and filter paper, respectively. Cellulose was active enough even after adsorption on lignocresols. Limited physical contact between solid substrates and immobilized cellulose due to the presence of lignocresol seems to lead to lower enzymatic activity for solid substrates. Hardwood lignocresol-immobilized cellulose exhibits slightly higher activity than softwood lignocresol-immobilized cellulose when the same amount of cellulose is used per gram lignocresol. Although cellulose activity gradually decreases with recycling, sufficient enzymatic activity, at least for hydrolyzing soluble substrates, remains that it can be reused.
机译：纤维素的成本是限制纤维素生物质的酶促水解的主要因素。因此，纤维素的固定化将是重要的进步。木质素酚是在环境温度下由木质纤维素材料和酚衍生物合成的基于木质素的官能酚聚合物。仅通过混合以产生水不溶性木质素酚纤维素复合物，就可以容易地通过软木和硬木lig-nocresol固定源自里氏木霉的纤维素。相对于游离纤维素，在羧甲基纤维素（CMC）和滤纸的水解中，固定在木质甲酚上的纤维素酶的酶促水解性能分别约为80-90％和30-50％。纤维素即使吸附在木质甲酚上也具有足够的活性。由于木质甲酚的存在，固体底物与固定化纤维素之间的有限的物理接触似乎导致固体底物的酶活性降低。当每克木质素甲酚使用相同量的纤维素时，硬木木质素甲酚固定化纤维素显示出比软木木质素甲酚固定化纤维素稍高的活性。尽管纤维素的活性随着再循环而逐渐降低，但是至少对于水解可溶性底物而言，足够的酶活性仍然可以重复使用。

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