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首页> 外文期刊>Key Engineering Materials >Effect of Pamidronate on Protein Adsorption and Osteoblast Adhesion to Hydroxyapatite Bioceramics Scaffold
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Effect of Pamidronate on Protein Adsorption and Osteoblast Adhesion to Hydroxyapatite Bioceramics Scaffold

机译：帕米膦酸对羟基磷灰石生物陶瓷支架的蛋白质吸附和成骨细胞粘附的影响

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摘要

This study is to investigate the effect of PAM on protein adsorption and osteoblastic cells adhesion to HA bioceramics. PAM was immobilized on the surface of HA bioceramics for bone scaffold by chelating. The outermost layer of the specimens was analyzed by XPS and FT-1R. The protein adsorption test was performed using the 10% bovine calf serum absorbed on the specimens in vitro. The osteoblastic cells were inoculated and cultured on the scaffolds. SEM, MTT test and ALP activity test evaluated the cell attachment, proliferation and activity on the scaffolds. Characteristic peaks in XPS and FT-IR spectra indicated PAM being immobilized on the surface of the bioceramics. PAGE and 2-D DIGE results indicated that HA absorbed more acidic proteins, while PAM-HA absorbed more basic and neutral proteins. The cell culture test indicated that the cells actively proliferated on the scaffolds. There was no significant difference between the ALP activity of the cells cultured for 1d, 3d, 5d and 7d on PAM-HA and that of the controls. PAM had no obvious effect on the cytocompatibility of HA, and PAM-HA bioceramics could be used as bone scaffold with potential ability to improve osteogenesis.
机译：这项研究旨在调查PAM对HA生物陶瓷对蛋白质吸附和成骨细胞粘附的影响。通过螯合将PAM固定在用于骨支架的HA生物陶瓷的表面上。标本的最外层用XPS和FT-1R分析。蛋白质吸附测试是使用体外在标本上吸收的10％牛犊血清进行的。接种成骨细胞并在支架上培养。 SEM，MTT测试和ALP活性测试评估了细胞在支架上的附着，增殖和活性。 XPS和FT-IR光谱中的特征峰表明PAM固定在生物陶瓷的表面上。 PAGE和2-D DIGE结果表明HA吸收了更多的酸性蛋白质，而PAM-HA吸收了更多的碱性和中性蛋白质。细胞培养测试表明细胞在支架上活跃增殖。在PAM-HA上培养1d，3d，5d和7d的细胞与对照组的ALP活性之间无显着差异。 PAM对HA的细胞相容性没有明显影响，并且PAM-HA生物陶瓷可以用作具有改善成骨能力的骨支架。

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