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首页> 外文期刊>The Korean journal of chemical engineering >Sugaring-out extraction of erythromycin from fermentation broth
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Sugaring-out extraction of erythromycin from fermentation broth

机译：从发酵液中加糖萃取红霉素

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This study reports the sugaring-out extraction of erythromycin from fermentation broth using acetonitrile (ACN) as solvent and glucose as a mass separating agent. Different process parameters-glucose concentration, temperature, ACN/water ratio and pH-were optimized to achieve maximum extraction of erythromycin. 88% (w/w) of erythromycin was extracted from the model system with following optimized conditions: glucose 156.3 g/L; temperature 4 degrees C; ACN/water ratio 1 and pH 8.3. Further, the effect of typical fermentation media components (starch, soybean flour, CaCO3, NaCl and (NH4)(2)SO4) on sugaring out extraction of erythromycin was also investigated. Starch, soybean flour and CaCO3 were observed to affect erythromycin extraction only at higher concentration. Removal of suspended solids from simulated as well as real broth prior to extraction enhanced the extraction efficiency (from 72% to 87%). Sugaring out extraction of erythromycin was found to be more effective than salting out extraction. Also, higher partition coefficient was achieved in the present work than other reported methods using carbohydrates as mass separating agent. Further, it was found that the antimicrobial activity of erythromycin was preserved during sugaring out extraction of erythromycin.
机译：本研究报告使用乙腈（ACN）作为溶剂和葡萄糖作为质量分离剂，从发酵液中加糖从发酵液中萃取。优化不同的工艺参数 - 葡萄糖浓度，温度，ACN /水比和pH-达到红霉素的最大提取。从模型系统中提取88％（w / w）的红霉素，在模型系统中提取，以下优化条件：葡萄糖156.3 g / l;温度4℃; ACN /水比1和pH 8.3。此外，还研究了典型发酵培养基组分（淀粉，大豆粉，CaCO 3，NaCl和（NH4）（2）SO 4）的效果也研究了加糖的饮用萃取。观察到淀粉，大豆粉和CaCO 3仅以较高浓度影响红霉素萃取。在提取之前从模拟和真实肉汤中除去悬浮固体，提高了提取效率（从72％到87％）。发现含有红霉素的提取，比盐萃取更有效。此外，在本工作中实现了比使用碳水化合物作为质量分离剂的其他报道的方法在本工作中实现的更高分配系数。此外，发现在加糖酶的萃取过程中，在红细胞的提取过程中保存了红细胞的抗微生物活性。

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