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首页> 外文期刊>Limnology >DNA extraction and amplification methods for ephippial cases of Daphnia resting eggs in lake sediments: a novel approach for reconstructing zooplankton population structure from the past
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DNA extraction and amplification methods for ephippial cases of Daphnia resting eggs in lake sediments: a novel approach for reconstructing zooplankton population structure from the past

机译：DNA提取和扩增方法在湖底沉积物水蚤的卵性海豚病例中：一种过去重建浮游动物种群结构的新方法

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摘要

This study describes a method of DNA extraction and amplification for ephippial cases of Daphnia resting eggs from lake sediment. Recent studies have reconstructed succession records of Daphnia species by genetically analyzing Daphnia resting eggs stored in lake sediments and revealed changes in dominant Daphnia species that correspond well with environmental changes. However, this approach is not applicable to lakes where most of the resting eggs in the sediment have already hatched out. We modified conventional methods for DNA extraction and amplification to enable genetic analyses of the ephippial case that envelops and protects the resting eggs, and we compared the performance of the modified method to the conventional one. We confirmed that we could efficiently analyze the sequences of the ephippial cases collected in a sediment core using our modified method. It enables us to reconstruct changes in genetic structure of Daphnia populations regardless of hatching rates of the resting eggs.
机译：这项研究描述了一种DNA提取和扩增方法，该方法可用于从湖底物中吸食海蚤的卵的ph虫病例。最近的研究通过对湖泊沉积物中储存的水蚤卵进行遗传分析，重建了水蚤物种的演替记录，并揭示了水蚤优势物种的变化与环境变化非常吻合。但是，这种方法不适用于沉积物中大部分静止卵已经孵化出来的湖泊。我们对DNA提取和扩增的常规方法进行了改进，以便能够对包裹和保护静止卵的以色皮病进行遗传分析，并且将改良方法的性能与常规方法进行了比较。我们证实，使用改进后的方法，我们可以有效地分析在沉积岩心中收集到的以太病例的序列。它使我们能够重建水蚤种群遗传结构的变化，而与静息卵的孵化率无关。

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  • 来源
    《Limnology》 |2012年第3期|p.261-267|共7页
  • 作者

    Seiji Ishida; Hajime Ohtsuki; Tamotsu Awano; Narumi K. Tsugeki; Wataru Makino; Yoshihisa Suyama; Jotaro Urabe;

  • 作者单位

    International Advanced Research and Education Organization, Tohoku University, Sendai, Miyagi, 980-8578, Japan;

    Graduate School of Life Sciences, Tohoku University, Seibutsu-tou 306, 6-3 Aramaki-aza-Aoba, Aoba-ku, Miyagi, Sendai, 980-8578, Japan;

    Graduate School of Life Sciences, Tohoku University, Seibutsu-tou 306, 6-3 Aramaki-aza-Aoba, Aoba-ku, Miyagi, Sendai, 980-8578, Japan;

    Senior Research Fellow Center, Ehime University, Matsuyama, 790-8577, Japan;

    Graduate School of Life Sciences, Tohoku University, Seibutsu-tou 306, 6-3 Aramaki-aza-Aoba, Aoba-ku, Miyagi, Sendai, 980-8578, Japan;

    Field Science Center, Graduate School of Agricultural Science, Tohoku University, Osaki, Miyagi, 989-6711, Japan;

    Graduate School of Life Sciences, Tohoku University, Seibutsu-tou 306, 6-3 Aramaki-aza-Aoba, Aoba-ku, Miyagi, Sendai, 980-8578, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Paleolimnology; Daphnia; Resting eggs; Ephippia; DNA extraction;

    机译：古生物学;水蚤;雌卵;麻;DNA提取;

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