• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Limnology >In-situ growth rate of Microcystis spp. and their growth-limiting factors: use of cellular RNA content
【24h】

In-situ growth rate of Microcystis spp. and their growth-limiting factors: use of cellular RNA content

机译：微囊藻的原位生长速率及其生长限制因素：利用细胞RNA含量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In 2003 and 2004, we conducted field investigations of a canal during the summer algal bloom to estimate the in-situ growth rate of Microcystis spp. and its limiting factors. Cellular RNA content (RNA/cell), determined by the real-time PCR method with a primer specific for amplification of Microcystis rRNA, was used as an index of in-situ growth rate. A Microcystis bloom was found in the canal in summer 2004 but not in summer 2003, because of its coldness. Corresponding to this, the average value of RNA/cell in 2004 was significantly higher than that in 2003. Water temperature, light intensity, and NO3 and PO4 concentrations were regarded as the factors limiting the in-situ growth rate of Microcystis in the canal, and their effects were quantified on the basis of laboratory experimental data. Effects of temperature and light intensity (photoinhibition by excessive photon flux density) were found to be important in limiting the growth rate, and more severe limitation was suggested in 2003. We then estimated the in-situ growth rate from the combined effect of these limiting factors. The estimated in-situ growth rates correlated significantly with RNA/cell in each year and in the combined (2003 + 2004) data. This agreement between our two different methods for estimation of in-situ growth rate suggests the validity of our approaches. This study was first field application of cellular RNA content as an index of algal growth rate in natural water samples.
机译：在2003年和2004年，我们对夏季藻华期间的一条运河进行了现场调查，以估算微囊藻的原位生长速率。及其限制因素。通过实时PCR方法，使用对微囊藻rRNA扩增具有特异性的引物测定的细胞RNA含量（RNA /细胞）用作原位生长速率的指标。 2004年夏季在运河中发现了微囊藻，但由于寒冷，没有发现。与此相应，2004年RNA /细胞的平均值明显高于2003年。水温，光照强度以及NO3 和PO4 的浓度被认为是限制原位的因素。根管内微囊藻的生长速率及其作用根据实验室实验数据进行定量。发现温度和光强度的影响（光子通量密度过大引起的光抑制）在限制生长速率方面很重要，并且在2003年提出了更严格的限制条件。然后，我们根据这些限制条件的联合作用来估算原位生长速率因素。在每年以及联合（2003 + 2004）数据中，估计的原位生长速率与RNA /细胞显着相关。我们两种不同的估计原位生长速度的方法之间的一致性表明了我们方法的有效性。这项研究是首次将细胞RNA含量作为天然水样品中藻类生长速率的指标进行现场应用。

著录项

  • 来源
    《Limnology》 |2011年第3期|p.235-243|共9页
  • 作者

    Takashi Nagai; Noriko Tomioka; Tatsuya Kawasaki; Akio Imai; Kazuo Matsushige;

  • 作者单位

    National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-0053, Japan;

    National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-0053, Japan;

    National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-0053, Japan;

    National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-0053, Japan;

    National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-0053, Japan;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    In-situ growth rate; Cellular RNA content; Microcystis; Limiting factor;

    机译：原位生长率;细胞RNA含量;微囊藻;限制因子;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号