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首页> 外文期刊>Acta Biotechnologica >The influence of growth rate and growth-limiting factors on the production of ice nuclei in pseudomonas syringae CCM 4073 in continuous culture
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The influence of growth rate and growth-limiting factors on the production of ice nuclei in pseudomonas syringae CCM 4073 in continuous culture

机译:生长速率和生长限制因子对丁香假单胞菌CCM 4073连续培养中冰核产生的影响

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摘要

The influence of different growth-limiting factors-namely the sources of carbon, nitrogen and phosphorus and the dilution (growth) rate-on the ice-nucleation activity of Pseudomonas syringae CCM 4073 was studied. A higher ice-nucleation activity wasobserved at a lower dilution (growth) rate (D = 0.1 h~(-1)) than at a higher dilution (growth) rate (D - 0.3 h~(-1)) Remarkable differences in icenucleation activity were found in its dependence on the growth-limiting factor. The highest ice-nucleation activity was observed under carbon limitation (T_90 -2.7 deg C), a medium activity under nitrogen limitation (T_90 =-5 deg C) and lowest activity under phosphorus limitation (T_90 = -12.3 deg C). After the addition of excess nitrogen or phosphorus to steady-state cultures, the ice-nucleation activity was restored.
机译:研究了不同的生长限制因子,即碳,氮和磷的来源以及稀释(生长)速率,对丁香假单胞菌CCM 4073的冰核活性的影响。在较低的稀释(生长)速率(D = 0.1 h〜(-1))下观察到较高的冰成核活性,而在较高的稀释(生长)速率(D-0.3 h〜(-1))下观察到发现其活性依赖于生长限制因子。在碳限制下(T_90 -2.7摄氏度)观察到最高的冰核活性,在氮限制下(T_90 = -5摄氏度)观察到中等的冰成核活性,而在磷限制下(T_90 = -12.3摄氏度)观察到最低的冰成核活性。向稳态培养物中添加过量的氮或磷后,冰核活性得以恢复。

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