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首页> 外文期刊>Marine biology >Bivalve transcriptomics reveal pathogen sequences and a powerful immune response of the Mediterranean mussel (Mytilus galloprovincialis)
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Bivalve transcriptomics reveal pathogen sequences and a powerful immune response of the Mediterranean mussel (Mytilus galloprovincialis)

机译：双壳类转录组学揭示了地中海贻贝（Mytilus galloprovincialis）的病原体序列和强大的免疫反应

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Bivalves have colonized the interface between land and sea for the last 500 million years. Although bivalves lack an adaptive immune system, they are extraordinarily well adapted to adverse environmental conditions. Bivalves are valuable aquaculture resources worldwide and are used as sentinels for monitoring pollution. In this work, the immune transcriptomes of mussels (Mytilus galloprovincialis and edulis) and clam (Ruditapes decussatus) were sequenced. For comparative purposes, an already published transcriptome dataset of Ruditapes philippinarum was also included in the analyses. The 454 pyrosequencing of stimulated hemocytes resulted in more than 400,000 reads for each transcriptome. The percentage of annotated sequences ranges from 50% for mussels to 30-40% for clams. Considering the 28,061 non-redundant sequences from the four transcriptomes, the four species share 785 genes. Moreover, sequences related to different putative pathogens were found in the four bivalves. A high number of bivalve herpesvirus ORFs were found, which confirms the value of NGSs as tools to detect and quantify pathogen RNA. Based on an examination of the immune-enriched transcriptomes of these four species, we can conclude that bivalves present an immune system that differs from its conventional characterization as a simple innate immune response against invading pathogens. Enrichment analyses showed that species in the Mytilus genus, especially M. galloprovincialis, possesses a significantly higher number of sequences related to immune processes and killing molecules than species in the Ruditapes genus. This could be related to the broader ecological niche occupied by mussels and the scarcity of reported mussel mass mortalities compared to the high number of mass mortalities reported for clams.
机译：在过去的5亿年中，双壳类已经在陆地和海洋之间的接口上殖民了。尽管双壳类动物缺乏适应性免疫系统，但它们非常适应恶劣的环境条件。双壳类是全世界宝贵的水产养殖资源，并被用作监测污染的前哨。在这项工作中，对贻贝（Mytilus galloprovincialis和可食）和蛤（Ruditapes decussatus）的免疫转录组进行了测序。为了进行比较，分析中还包括了已出版的菲律宾蛤仔的转录组数据集。刺激的血细胞的454焦磷酸测序导致每个转录组读取超过40万次。带注释的序列的百分比范围从贻贝的50％到蛤的30-40％。考虑到来自四个转录组的28,061个非冗余序列，这四个物种共有785个基因。此外，在四个双壳类动物中发现了与不同推定病原体相关的序列。发现大量双壳疱疹病毒ORF，这证实了NGS作为检测和定量病原体RNA的工具的价值。基于对这四个物种的富含免疫的转录组的检查，我们可以得出结论，双壳类动物呈现的免疫系统不同于其常规特征，即针对入侵病原体的简单先天免疫应答。富集分析表明，Mytilus属中的物种，特别是Galloprovincialis物种，与Ruditapes属中的物种相比，具有与免疫过程和杀死分子有关的序列数量要多得多。这可能与贻贝所占据的更广泛的生态位以及报道的贻贝大规模死亡相比报道的蛤类大量死亡的数量稀少有关。

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  • 来源
    《Marine biology》 |2018年第4期|61.1-61.20|共20页
  • 作者

    Moreira Rebeca; Balseiro Pablo; Forn-Cuni Gabriel; Milan Massimo; Bargelloni Luca; Novoa Beatriz; Figueras Antonio;

  • 作者单位

    CSIC, IIM, Eduardo Cabello 6, Vigo 36208, Spain;

    CSIC, IIM, Eduardo Cabello 6, Vigo 36208, Spain;

    CSIC, IIM, Eduardo Cabello 6, Vigo 36208, Spain;

    Univ Padua, Dept Comparat Biomed & Food Sci, Legnaro, PD, Italy;

    Univ Padua, Dept Comparat Biomed & Food Sci, Legnaro, PD, Italy;

    CSIC, IIM, Eduardo Cabello 6, Vigo 36208, Spain;

    CSIC, IIM, Eduardo Cabello 6, Vigo 36208, Spain;

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