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Is metabarcoding suitable for estuarine plankton monitoring? A comparative study with microscopy

机译：元条形码是否适合河口浮游生物监测？显微镜的比较研究

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摘要

Metabarcoding is becoming an increasingly valuable alternative approach to biodiversity assessment, due to the combination of extreme sensitivity and potential for the highest taxonomic resolution in a cost-and time-effective methodology. To evaluate the capacity of metabarcoding for estuarine plankton monitoring, a comparison between the results obtained with this approach were compared with those based on traditional taxonomic analysis (microscopy). Database incompleteness, one of the main limitations of metabarcoding, was somewhat overcome by the addition of DNA sequences for local species, which increased the taxonomic assignment success from 23.7 to 50.5 %. When the communities were studied along with environmental variables, similar spatial and temporal trends of taxonomic diversity were observed for metabar-coding and microscopic studies of zooplankton, but not for phytoplankton. This is most likely attributable to the lack of representative sequences for phytoplankton species in current databases. In addition, there was high correspondence in community composition when comparing abundances estimated from metabarcoding and microscopy, suggesting semiquantitative potential for metabarcoding. Furthermore, metabarcoding allowed the detection and identification of two non-indigenous species (NIS) found in the study area at abundances hardly detectable by microscopy. Overall, our results indicate that metabarcoding is a powerful approach with excellent possibilities for use in plankton monitoring, early detection of NIS and plankton biodiversity shifts.
机译：由于具有极高的成本敏感性和时间效益的方法具有极高的敏感性和最高的生物分类学分辨率，因此元条形码正在成为生物多样性评估中一种越来越有价值的替代方法。为了评估元条形码在河口浮游生物监测中的能力，将使用该方法获得的结果与基于传统分类学分析（显微镜）的结果进行了比较。数据库不完整是元条形码的主要限制之一，通过添加本地物种的DNA序列可以部分克服，这将分类分配成功率从23.7％提高到了50.5％。当对群落和环境变量进行研究时，对于浮游动物的metabar编码和微观研究，观察到了相似的生物多样性多样性时空趋势。这很可能是由于当前数据库中缺乏浮游植物物种的代表性序列。此外，比较元条形码和显微镜检查估计的丰度时，群落组成具有高度对应性，表明元条形码的半定量潜力。此外，元条形码技术可以检测和鉴定在研究区域中发现的两种非土著物种（NIS），其数量很难用显微镜检测到。总体而言，我们的结果表明，元条形码是一种功能强大的方法，极有可能用于浮游生物监测，早期发现NIS和浮游生物的生物多样性转移。

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  • 来源
    《Marine biology》 |2016年第7期|149.1-149.13|共13页
  • 作者

    Abad David; Albaina Aitor; Aguirre Mikel; Laza-Martinez Aitor; Uriarte Ibon; Iriarte Arantza; Villate Fernando; Estonba Andone;

  • 作者单位

    Univ Basque Country, UPV EHU, Dept Genet Phys Anthropol & Anim Physiol, Genom Resources Grp, Leioa, Spain;

    Univ Basque Country, UPV EHU, Dept Genet Phys Anthropol & Anim Physiol, Genom Resources Grp, Leioa, Spain|Vitoria Gasteiz City Council, Environm Studies Ctr CEA, Vitoria, Spain;

    Univ Basque Country, UPV EHU, Dept Genet Phys Anthropol & Anim Physiol, Genom Resources Grp, Leioa, Spain;

    Univ Basque Country, UPV EHU, Dept Plant Biol & Ecol, Phytoplankton Grp, Leioa, Spain;

    Univ Basque Country, UPV EHU, Dept Plant Biol & Ecol, Zooplankton Grp,Lab Ecol Zooplankton, Gasteiz, Spain;

    Univ Basque Country, UPV EHU, Dept Plant Biol & Ecol, Zooplankton Grp,Lab Ecol Zooplankton, Gasteiz, Spain;

    Univ Basque Country, UPV EHU, Dept Plant Biol & Ecol, Zooplankton Grp,Lab Ecol Zooplankton, Gasteiz, Spain;

    Univ Basque Country, UPV EHU, Dept Genet Phys Anthropol & Anim Physiol, Genom Resources Grp, Leioa, Spain;

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