Determining the diet of larvae of the red rock lobster (Jasus edwardsii) using high-throughput DNA sequencing techniques

R. ORorke; S. D. Lavery; M. Wang; S. D. Nodder; A. G. Jeffs

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Determining the diet of larvae of the red rock lobster (Jasus edwardsii) using high-throughput DNA sequencing techniques

机译：使用高通量DNA测序技术确定红岩龙虾（Jasus edwardsii）幼虫的饮食

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The diet of Jasus edwardsii during its ～2-year larval (phyllosoma) phase is largely unknown. High mortalities experienced during larviculture might be reduced if their diet were nutritionally modelled on the natural diet. Here, prey species were identified from phyllosoma midgut glands using 454 pyrosequencing of 18S rDNA. We found that gelatinous zooplankton, particularly Siphonophora and Ctenophora, occurred frequently in the midgut glands of phyllosomas, resolving previous conjecture that these animals are in the diet of J. edwardsii phyllosomas. A high occurrence of sequencing reads from unicellular microbes may also reflect a reliance on scavenging detritus.

机译：在大约2年的幼虫期（叶状体）阶段，爱德华猪的饮食是未知的。如果以自然饮食为营养模型，则可以减少幼虫养殖期间的高死亡率。在这里，使用454焦磷酸测序18S rDNA从食叶中肠腺中鉴定出猎物。我们发现，胶状浮游动物，特别是虹吸虫和蓝鳍金枪鱼，经常发生在叶状瘤的中肠，解决了以前的推测，即这些动物是在爱德华氏疟疾的饮食中。来自单细胞微生物的测序读数的高发生率也可能反映了对清除碎屑的依赖。

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《Marine biology》 |2014年第3期|551-563|共13页
作者
R. ORorke; S. D. Lavery; M. Wang; S. D. Nodder; A. G. Jeffs;
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School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand;

School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand ,Institute of Marine Science, University of Auckland, P.O. Box 349, Warkworth 0941, New Zealand;

Institute of Marine Science, University of Auckland, P.O. Box 349, Warkworth 0941, New Zealand;

National Institute of Water and Atmospheric Research (NIWA) Ltd., Private Bag 14-901, Kilbirnie, Wellington 6021, New Zealand;

Institute of Marine Science, University of Auckland, P.O. Box 349, Warkworth 0941, New Zealand;

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Determining the diet of larvae of the red rock lobster (Jasus edwardsii) using high-throughput DNA sequencing techniques

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