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首页> 外文期刊>Marine biology >Bacterial communities of the marine sponges Hymeniacidon heliophila and Polymastia janeirensis and their environment in Rio de Janeiro, Brazil
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Bacterial communities of the marine sponges Hymeniacidon heliophila and Polymastia janeirensis and their environment in Rio de Janeiro, Brazil

机译：巴西里约热内卢海棉Hymeniacidon heliophila和Polymastia janeirensis的细菌群落及其环境

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In this study we performed a survey of the bacterial communities associated with the Western Atlantic demosponges Hymeniacidon heliophila and Polymastia janeirensis, based on 16S rRNA sequencing and transmission electron microscopy (TEM). We compared diversity and composition of the sponge-associated bacteria to those of environmental bacteria, represented by free-living bacte-rioplankton and by bacteria attached to organic particulate matter in superficial sediments. Partial bacterial 16S rRNA sequences from seawater, sediment, and sponges were retrieved by PCR, cloning, and sequencing. Sequences were subjected to rarefaction analyses, phylogenetic tree construction, and LIBSHUFF quantitative statistics to verify coverage and similarity between libraries. Community structure of the free-living bacterioplankton was phylogeneticaHy different from that of the sponge-associated bacterial assemblages. On the other hand, some sediment-attached bacteria were also found in the sponge bacterial community, indicating that sponges may incorporate bacteria together with sediment particles. Rare and few prokaryotic morphotypes were found in TEM analyses of sponge meso-hyl matrix of both species. Molecular data indicate that bacterial richness and diversity decreases from bacterioplankton, to particulate organic sediment, and to H. heliophila and P. janeirensis. Sponges from Rio de Janeiro harbor a pool of novel and exclusive sponge-associated bacterial taxa. Sponge-associated bacterial communities are composed of both taxons shared by many sponge groups and by species-specific bacteria.
机译：在这项研究中，我们基于16S rRNA测序和透射电子显微镜（TEM）对与西大西洋双节菌Hemenophila和Polymastia janeirensis相关的细菌群落进行了调查。我们将海绵相关细菌的多样性和组成与环境细菌的多样性和组成进行了比较，以自由活动的浮游浮游生物和附着在表层沉积物中有机颗粒物的细菌为代表。通过PCR，克隆和测序检索了海水，沉积物和海绵中的部分细菌16S rRNA序列。对序列进行稀疏分析，系统树构建和LIBSHUFF定量统计，以验证文库之间的覆盖率和相似性。自由生活的浮游生物的群落结构与海绵相关的细菌群落在系统发育上是不同的。另一方面，在海绵细菌群落中也发现了一些附着有沉积物的细菌，这表明海绵可能会将细菌与沉积物颗粒结合在一起。在这两种物种的海绵介观基体的TEM分析中，很少见到极少的原核形态。分子数据表明，细菌的丰富度和多样性从浮游细菌到颗粒状有机沉积物，再到嗜血嗜血菌和詹氏疟原虫，都在减少。来自里约热内卢的海绵拥有大量新颖且与海绵相关的细菌类群。与海绵相关的细菌群落由许多海绵类和特定物种的细菌共享的分类单元组成。

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