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首页> 外文期刊>Marine ecology >Concordance of microsatellite and mitochondrial DNA markers in detecting genetic population structure in the boring giant clam Tridacna crocea across the Indo-Malay Archipelago
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Concordance of microsatellite and mitochondrial DNA markers in detecting genetic population structure in the boring giant clam Tridacna crocea across the Indo-Malay Archipelago

机译：微卫星和线粒体DNA标记在检测印度马来群岛无聊的大蛤d大鳄中的遗传种群结构中的一致性

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摘要

Mitochondrial DNA (mtDNA) is a single, usually non-recombining locus, and often uniparentally inherited. Therefore, its ability to reveal recent gene flow among populations is usually questioned. In this study, the genetic population structure of 16 populations of Tridacna crocea (n=366) from the Indo-Malay Archipelago (IMA) was examined with 10 microsatellite markers and compared to previous studies using mtDNA, in order to test if the revealed population structure was congruent between the two marker systems. The results showed that the genetic population structure revealed by the two marker systems was mostly congruent, with a high correlation between cytochrome c oxidase subunit I (COI) and microsatellites. The studied populations could be divided by both marker systems as follows: (i) Eastern Indian Ocean, (ii) Central IMA, and (iii) Western Pacific. Populations in the Central IMA showed high gene flow. However, populations in the Java Sea (Karimunjava, Pulau Seribu) were grouped into a separate cluster by mtDNA analysis, while this grouping was not detected by microsatellites. It was also noteworthy that there is obvious heterozygosity deficiency in most of the populations, which may be caused by null alleles, inbreeding or population expansion. Overall, these results indicate that the mitochondrial COI gene is applicable for population genetic analysis and precise recovery of connectivity patterns of giant clams. Therefore, the combination of mtDNA and nuclear DNA markers can lead to a more complete understanding of population genetics. Moreover, this study is expected to facilitate fully displaying the population genetic structure of giant clams combining with other researchers' results.
机译：线粒体DNA（mtDNA）是一个单一的，通常是非重组的基因座，并且通常是单亲遗传的。因此，通常它是否具有揭示群体间最新基因流动的能力。在这项研究中，使用10个微卫星标记检查了来自印度－马来群岛（IMA）的16个大戟Tri（n = 366）的遗传种群结构，并将其与以前使用mtDNA进行的研究进行比较，以测试是否发现了所揭示的种群两个标记系统之间的结构一致。结果表明，两个标记系统揭示的遗传种群结构基本一致，细胞色素C氧化酶亚基I（COI）与微卫星之间的相关性很高。可以通过两种标记系统对研究种群进行如下划分：（i）东印度洋，（ii）中部IMA，以及（iii）西太平洋。中部IMA的种群显示高基因流。但是，通过mtDNA分析，将爪哇海（Karimunjava，Seribu岛）的种群分为一个单独的簇，而微卫星却没有检测到这种分组。还值得注意的是，大多数人群中均存在明显的杂合性缺陷，这可能是由无效等位基因，近交或种群扩展引起的。总体而言，这些结果表明线粒体COI基因适用于群体遗传分析和大蛤的连接模式的精确恢复。因此，将mtDNA和核DNA标记结合使用可以更全面地了解种群遗传学。此外，该研究有望与其他研究人员的研究结果相结合，以促进充分展示大蛤的种群遗传结构。

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