• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Marine ecology >Can light intensity cause shifts in natural product and bacterial profiles of the sponge Aplysina aerophoba?
【24h】

Can light intensity cause shifts in natural product and bacterial profiles of the sponge Aplysina aerophoba?

机译：光照强度会导致天然产物和Apersina aerophoba海绵的细菌分布发生变化吗？

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The potential role of microbial consortia on sponge chemistry is well known. However, how environmental factors affect microbial and chemical profiles and how these shifts affect the sponge holobiont are far from being understood. This study experimentally investigated the effect of light on both the concentration of secondary metabolites and the bacterial assemblages of the sponge Aplysina aerophoba. We quantified major brominated alkaloids (BAs) using a high-performance liquid chromatography system coupled with a UV-detector and analysed sponge-associated bacteria using denaturing gradient gel electrophoresis of 16S rRNA gene amplicons. We identified distinct chemical and bacterial profiles between the ectosome and the choanosome of A.aerophoba. The abundance of most secondary metabolites increased regardless of the illumination regime. We found that the probability of occurrence of three microbial phylotypes (operational taxonomic units 84, 86 and 87) was strongly associated with increasing concentrations of three brominated compounds (aerophobin-1, aplysinamisin-1 and isofistularin-3). Although the role of these bacteria remains uninvestigated, these associations between natural products and specific microbial phylotypes outline further hypotheses that will improve our understanding of the organization and functioning of these complex host–symbiont interactions.
机译：微生物联盟对海绵化学的潜在作用是众所周知的。但是，环境因素如何影响微生物和化学特性以及这些变化如何影响海绵状全灭臭剂尚不清楚。这项研究通过实验研究了光对次生代谢产物浓度和拟南芥Aperophina aerophoba细菌组成的影响。我们使用高效液相色谱系统结合紫外线检测器对主要溴化生物碱（BAs）进行了定量，并使用变性梯度凝胶电泳对16S rRNA基因扩增子进行了海绵相关细菌的分析。我们确定了A.aophophoba的胞外体和choanosome之间不同的化学和细菌概况。不论光照方案如何，大多数次生代谢产物的丰度都会增加。我们发现三种微生物系统型（操作分类单位84、86和87）的发生概率与三种溴化化合物（aerophobin-1，aplysinamisin-1和isofistularin-3）浓度的增加密切相关。尽管这些细菌的作用尚待研究，但天然产物与特定微生物系统型之间的这些关联概述了进一步的假设，这些假设将增进我们对这些复杂的宿主-共生体相互作用的组织和功能的理解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号