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Chemical-bonding states and protein adsorptions on ozone/UV treated polystyrene films

机译：臭氧/紫外线处理的聚苯乙烯薄膜上的化学键态和蛋白质吸附

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摘要

We have studied on chemical bonding states on polystyrene (PS) films treated by ozone aeration under UV light irradiation (ozone/UV treatment) in distilled water and in an aqueous ammonia solution, and on adsorption behavior of bovine serum albumin (BSA) and bovine serum y globulin (BGG) on the treated PS film. X-ray photoelectron spectroscopy (XPS) surface analysis indicates that the ozone/UV treatment induces ring-opening reactions of benzene rings in PS molecules, introducing C-O and C=O bonds on the PS film treated in distilled water and C-O, C-O, and N-H bonds on the film treated in the aqueous ammonia solution. Moreover, the adsorption of BSA having negative surface charge on the treated PS film rapidly increases with an increase in treatment time of the ozone/UV treatment, reaching the maximum in the 2-hour treatment. Despite an increase in treatment time, on the contrary, the adsorption of BGG, of which the surface charge is neutral, is almost constant. From these results, we consider that the ozone/UV treatment is more effective for the BSA adsorption on the PS film.
机译：我们已经研究了在臭氧水，蒸馏水和氨水溶液中在紫外线照射下（臭氧/紫外线处理）臭氧曝气处理的聚苯乙烯（PS）薄膜的化学键合状态，以及牛血清白蛋白（BSA）和牛的吸附行为经处理的PS膜上的血清y球蛋白（BGG）。 X射线光电子能谱（XPS）表面分析表明，臭氧/紫外线处理会引起PS分子中苯环的开环反应，从而在经蒸馏水处理的PS膜上引入CO和C = O键，并且CO，CO和在氨水溶液中处理过的薄膜上有NH键。而且，随着臭氧/ UV处理的处理时间的增加，在处理过的PS膜上具有负表面电荷的BSA的吸附迅速增加，在2小时处理中达到最大值。相反，尽管处理时间增加，但是表面电荷为中性的BGG的吸附几乎恒定。根据这些结果，我们认为臭氧/紫外线处理对于BSA在PS膜上的吸附更有效。

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