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首页> 外文期刊>Materials Science and Engineering >Electrochemical detection of L-dopa using crude Polyphenol oxidase enzyme immobilized on electrochemically reduced RGO-Ag nanocomposite modified graphite electrode
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Electrochemical detection of L-dopa using crude Polyphenol oxidase enzyme immobilized on electrochemically reduced RGO-Ag nanocomposite modified graphite electrode

机译：电化学还原RGO-Ag纳米复合修饰石墨电极上固定的粗制多酚氧化酶对L-多巴的电化学检测

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摘要

In the present work, we report the selective and sensitive electrochemical detection of L-dopa using crude Polyphenol oxidase enzyme (PPO) immobilized on electrochemically reduced graphene oxide–silver nanoparticles (RGO-Ag) nano composite modified graphite (Gr) electrode. The Gr electrode is first modified through the electrochemical reduction of graphene oxide - silver (GO-Ag) nano composite to RGO-Ag nano composite on graphite electrode. The crude extract of PPO fromManilkara Zapota(sapota) fruit is later immobilized onto Gr/RGO-Ag modified electrode. Modification of Gr electrode at each step is confirmed by cyclic voltammetry (CV) and Electrochemical impedance (EIS) techniques. The surface characterization of modified electrode is performed by Scanning electron microscopy (SEM). The developed sensor exhibited excellent electrocatalytic activity towards the detection of L-dopa in the concentration range of 1–150 µM with low detection limit and high sensitivity. Further, the practical applicability of the developed biosensor is examined for the detection of L-dopa in urine samples.
机译：在当前的工作中，我们报告了使用固定在电化学还原的氧化石墨烯-银纳米颗粒（RGO-Ag）纳米复合修饰石墨（Gr）电极上的粗制多酚氧化酶（PPO）对左旋多巴进行选择性和灵敏的电化学检测。首先通过石墨烯氧化物-银（GO-Ag）纳米复合材料的电化学还原为石墨电极上的RGO-Ag纳米复合材料来修饰Gr电极。随后将来自Manilkara Zapota（sapota）果实的PPO粗提物固定在Gr / RGO-Ag修饰的电极上。通过循环伏安法（CV）和电化学阻抗（EIS）技术确认了在每个步骤中对Gr电极的修饰。修饰电极的表面表征通过扫描电子显微镜（SEM）进行。研发的传感器在1–150µµM的浓度范围内对L-多巴的检测表现出出色的电催化活性，具有低检测限和高灵敏度。此外，检查了开发的生物传感器的实际适用性以检测尿液样本中的左旋多巴。

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