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Molecularly imprinted fluorescent probe based on FRET for selective and sensitive detection of doxorubicin

机译:基于FRET的分子印迹荧光探针可选择性,灵敏地检测阿霉素

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摘要

In this work, a new type of fluorescent probe for detection of doxorubicin has been constructed by the combined use of fluorescence resonance energy transfer (FRET) technology and molecular imprinting technique (MIT). Using doxorubicin as the template, the molecularly imprinted polymer thin layer was fabricated on the surfaces of carbon dot (CD) modified silica by sol-gel polymerization. The excitation energy of the fluorescent donor (CDs) could be transferred to the fluorescent acceptor (doxorubicin). The FRET based fluorescent probe demonstrated high sensitivity and selectivity for doxorubicin. The detection limit was 13.8 nM. The fluorescent probe was successfully applied for detecting doxorubicin in doxorubicin-spiked plasmas with a recovery of 96.8-103.8%, a relative standard deviation (RSD) of 1.3-2.8%. The strategy for construction of FRET-based molecularly imprinted materials developed in this work is very promising for analytical applications.
机译:在这项工作中,通过结合使用荧光共振能量转移(FRET)技术和分子印迹技术(MIT),构建​​了一种用于检测阿霉素的新型荧光探针。以阿霉素为模板,通过溶胶-凝胶聚合法在碳点(CD)修饰的二氧化硅表面上制备了分子印迹聚合物薄层。荧光供体(CD)的激发能可以转移到荧光受体(阿霉素)上。基于FRET的荧光探针显示出对阿霉素的高灵敏度和选择性。检测限为13.8 nM。该荧光探针已成功用于检测阿霉素加标血浆中的阿霉素,回收率为96.8-103.8%,相对标准偏差(RSD)为1.3-2.8%。在这项工作中开发的基于FRET的分子印迹材料的构建策略对于分析应用程序非常有希望。

著录项

  • 来源
    《Materials Science and Engineering》 |2017年第4期|31-39|共9页
  • 作者单位

    College of Chemistry and Materials Science, Hengyang Normal University, Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China;

    College of Chemistry and Materials Science, Hengyang Normal University, Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China;

    College of Chemistry and Materials Science, Hengyang Normal University, Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China;

    Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, PR China;

    College of Chemistry and Materials Science, Hengyang Normal University, Key Laboratory of Functional Organometallic Materials of Hunan Province University, Hengyang 421008, PR China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Doxorubicin; Molecular imprinting; FRET; Molecular recognition; Fluorescent carbon dots;

    机译:阿霉素分子印迹烦恼;分子识别;荧光碳点;

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