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首页> 外文期刊>Materials science & engineering >Synthesis of polymer-functionalized nanoscale graphene oxide with different surface charge and its cellular uptake, biosafety and immune responses in Raw264.7 macrophages
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Synthesis of polymer-functionalized nanoscale graphene oxide with different surface charge and its cellular uptake, biosafety and immune responses in Raw264.7 macrophages

机译：Raw264.7巨噬细胞中具有不同表面电荷的聚合物功能化纳米级氧化石墨烯的合成及其细胞摄取，生物安全性和免疫反应

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摘要

Polymer-functionalized graphene oxide (GO) has superior properties such as large surface area, extraordinary mechanical strength, high carrier mobility, good stability in physiological media and low cytotoxicity, making it an attractive material for drug and gene delivery. Herein, we successfully synthesized GO with an average size of 168.3 nm by a modified Hummers' method. Branched polyethylenimine (PEI) and 6-armed polyethylene glycol (PEG) functionalized GO complexes (GO-PEI and GO-PEG) with different zeta potentials of 47.2 mV and - 43.0 mV, respectively, were successfully synthesized through amide linkages between the COOH groups of GO and the NH_2 groups of PEI and PEG. Then, the interactions between GO-PEI and GO-PEG complexes and Raw264.7 mouse monocyte-macrophage cells were investigated. The GO-PEI and GO-PEG complexes could both be internalized by Raw264.7 cells. However, compared with the GO-PEG complex, the GO-PEI complex showed higher intracellular delivery efficiency in Raw264.7 cells. Moreover, it was found that the GO-PEI complex not only gathered in endosomes but also in the cytoplasm, whereas GO-PEG gathered in endosomes only. The MTT tests showed that both GO-PEI and GO-PEG complexes exhibited very low cytotoxicity towards Raw264.7 cells when at a low concentration. The cellular immune response test demonstrated the GO-PEG complex enhanced the secretion of IL-6, illustrating it was more stimulus towards macrophage cells. The above results indicated that the GO-PEI complex, with a positive surface charge, demonstrated better potential to be used in effective drug and gene delivery.
机译：聚合物功能化氧化石墨烯（GO）具有优异的性能，例如大表面积，非凡的机械强度，高载流子迁移率，在生理介质中的良好稳定性和低细胞毒性，使其成为用于药物和基因递送的诱人材料。在本文中，我们通过改进的Hummers方法成功合成了平均大小为168.3 nm的GO。通过COOH基团之间的酰胺键成功合成了具有不同zeta电位分别为47.2 mV和-43.0 mV的支化聚乙烯亚胺（PEI）和6臂聚乙二醇（PEG）功能化的GO配合物（GO-PEI和GO-PEG） GO和PEI和PEG的NH_2基团。然后，研究了GO-PEI和GO-PEG复合物与Raw264.7小鼠单核巨噬细胞之间的相互作用。 GO-PEI和GO-PEG复合物均可被Raw264.7细胞内化。然而，与GO-PEG复合物相比，GO-PEI复合物在Raw264.7细胞中显示出更高的细胞内递送效率。此外，发现GO-PEI复合物不仅聚集在内体中，而且还聚集在细胞质中，而GO-PEG仅聚集在内体中。 MTT测试表明，低浓度时，GO-PEI和GO-PEG复合物均对Raw264.7细胞表现出非常低的细胞毒性。细胞免疫应答测试表明，GO-PEG复合物增强了IL-6的分泌，说明它对巨噬细胞具有更多的刺激作用。以上结果表明，具有正表面电荷的GO-PEI复合物具有更好的潜力可用于有效的药物和基因递送。

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  • 来源
    《Materials science & engineering》 |2018年第9期|514-522|共9页
  • 作者

    Bing Wang; Xiaopeng Su; Junlong Liang; Lifeng Yang; Qinli Hu; Xinyi Shan; Junmin Wan; Zhiwen Hu;

  • 作者单位

    Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China;

    Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China;

    Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China;

    Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China;

    Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China;

    Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou 310018, China;

    Department of Polymer Materials, Zhejiang Sci-Tech University, Hangzhou 310018, China;

    Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, Zhejiang Sci-Tech University, Hangzhou 310018, China;

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  • 关键词

    GO-PEI; GO-PEG; Raw264.7 cell; Cellular uptake; Cytotoxicity; Immune response;

    机译：GO-PEI;聚乙二醇Raw264.7细胞;细胞摄取;细胞毒性;免疫反应;

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