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首页> 外文期刊>Materials science & engineering >Promoting effect of nano hydroxyapatite and vitamin D3 on the osteogenic differentiation of human adipose-derived stem cells in polycaprolactone/ gelatin scaffold for bone tissue engineering
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Promoting effect of nano hydroxyapatite and vitamin D3 on the osteogenic differentiation of human adipose-derived stem cells in polycaprolactone/ gelatin scaffold for bone tissue engineering

机译：纳米羟基磷灰石和维生素D3对聚己内酯/明胶支架用于骨骼组织工程的人脂肪干细胞成骨分化的促进作用

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摘要

Tissue engineering knowledge is a step towards the treatment of irreversible damages to human beings. In the present study, PCL/Gel, PCL/GelHA, PCL/Gel/Vit D3 and PCL/GelHA/Vit D3 (Polycaprolactone/Gelatin/Nanohydroxyapatite/Vitamin D3) composite scaffolds were successfully constructed using electrospinning method. The proliferation and differentiation of hADSCs into the bone phenotype were determined using MTT method, ALP activity, Von Kossa and Alizarin red staining, and qRT-PCR test. The simultaneous presence of nHA and vitamin D3 led to the increased activity of ALP in the early stages (on the 14th day) and increased mineralization in the late stages (on the 21st day) in differentiated hADSCs. Further, it was found that the use of nHA and vitamin D3 resulted in increased expression of BGLAP and COLL I and reduced expression of ALP and RUNX2 in hADSCs for 21 days. The results indicated that nHA and vitamin D3 have a synergistic effect on the osteogenic differentiation of hADSCs.
机译：组织工程知识是迈向治疗不可逆转的人类伤害的一步。在本研究中，成功​​地使用电纺丝方法构建了PCL / Gel，PCL / Gel / nHA，PCL / Gel / Vit D3和PCL / Gel / nHA / Vit D3（聚己内酯/明胶/纳米羟基磷灰石/维生素D3）复合支架。使用MTT法，ALP活性，Von Kossa和茜素红染色以及qRT-PCR测试确定hADSCs向骨表型的增殖和分化。 nHA和维生素D3的同时存在导致分化的hADSCs在早期（第14天）的ALP活性增加，在后期（第21天）的矿化增加。此外，发现使用nHA和维生素D3导致hADSCs中BGLAP和COLL I的表达增加，并且ALP和RUNX2的表达减少21天。结果表明，nHA和维生素D3对hADSCs的成骨分化具有协同作用。

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