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首页> 外文期刊>Materials science & engineering >Pre-osteoblast cell colonization of porous silicon substituted hydroxyapatite bioceramics: Influence of microporosity and macropore design
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Pre-osteoblast cell colonization of porous silicon substituted hydroxyapatite bioceramics: Influence of microporosity and macropore design

机译：多孔硅取代羟基磷灰石生物陶瓷的成骨细胞前定植：微孔和大孔设计的影响

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摘要

Silicate-substituted hydroxyapatite scaffolds containing multiscale porosity are manufactured. Model parts containing macropores of five cross-sectional geometries (circle, square, rhombus, star and triangle) and two sizes are shaped by microstereolithography. Three open microporosity contents (0.5, 23 or 37 vol%) are introduced in the ceramic. MC3T3-E1 pre-osteoblasts are seeded onto these scaffolds. Analysis of cell colonization inside the macropores after 7 and 14 days of cultivation shows that the cellular filling is proportional to the macropore size and strongly influenced by macropore shape. Straight edges and convex surfaces are detrimental. High aspect ratios, the absence of reentrant angles and the presence of acute angles, by creating concavities and minimizing flat surfaces, facilitate cell colonization. Rhombus and triangle cross-sections are thus particularly favorable, while square and star geometries are the least favored. An increase in the microporosity content strongly impairs cell growth in the macropores. The data are statistically analyzed using a principal components analysis that shows that macro- and microtopographical parameters of scaffolds must be collectively considered with correlated interactions to understand cell behavior. The results indicate the important cell sensing of topography during the initial step of cell adhesion and proliferation and evidence the need for an optimized scaffold design.
机译：制造具有多尺度孔隙率的硅酸盐取代的羟基磷灰石支架。包含五个横截面几何形状（圆形，正方形，菱形，星形和三角形）和两种尺寸的大孔的模型零件通过微立体光刻法成形。在陶瓷中引入了三个开放的微孔含量（0.5、23或37 vol％）。将MC3T3-E1前成骨细胞播种到这些支架上。培养7天和14天后，大孔内部的细胞定殖分析表明，细胞填充与大孔大小成正比，并受大孔形状的强烈影响。直边和凸面是有害的。高深宽比，不存在凹角和锐角，通过产生凹面并使平面最小化，有助于细胞定殖。因此，菱形和三角形的横截面特别有利，而方形和星形的几何形状最不受欢迎。微孔含量的增加强烈损害了大孔中的细胞生长。使用主成分分析对数据进行统计分析，结果表明必须共同考虑支架的宏观和微观形貌参数以及相关的相互作用，才能理解细胞行为。结果表明，在细胞粘附和增殖的初始步骤中，重要的细胞形貌是细胞学检测，并证明需要优化的支架设计。

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