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首页> 外文期刊>Materials science & engineering >Bioactive silica nanoparticles with calcium and phosphate for single dose osteogenic differentiation
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Bioactive silica nanoparticles with calcium and phosphate for single dose osteogenic differentiation

机译：具有钙和磷酸盐的生物活性二氧化硅纳米粒子，可用于单剂量成骨分化

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摘要

The differentiation of adult stem cells is usually performed in vitro, by exposing them to specific factors. Alternatively, one can use nanocarriers containing such factors, to be internalized by the cells. In this work we have reduce the size of those carriers to the nanoscale, developing bioactive silica nanoparticles with diameters under 100 nm, containing calcium and phosphate ions (SiNPs-CaP). These ions, once released inside adult stem cells, induce bone cell proliferation and differentiation, and stimulate the expression of bone-related proteins in a single dose administration. The SiNPs-CaP nanomaterials were prepared through a sol-gel approach, and the ions added with a post-synthesis functionalization method. The synthesized SiNPs-CaP have narrow size distribution, good colloidal stability, and show high levels of ion incorporation. Furthermore, the SiNPs-CaP have good cytocompatibility and promote the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSC), with alkaline phosphatase, osteopontin and osteocalcin production levels comparable to the ones obtained in standard osteogenic medium. The novel bioactive SiNPs-CaP are synthesized in a simple and fast manner and show the ability to promote osteogenic differentiation after a single dose administration, independently from external osteogenic inducers, showing great potential as carriers in bone tissue engineering applications.
机译：成人干细胞的分化通常是在体外进行，方法是将其暴露于特定因素下。或者，可以使用包含此类因子的纳米载体，以使其被细胞内在化。在这项工作中，我们已将这些载体的尺寸减小至纳米级，开发了直径小于100 nm的含有钙和磷酸根离子（SiNPs-CaP）的生物活性二氧化硅纳米颗粒。这些离子一旦在成人干细胞中释放，就会诱导骨细胞增殖和分化，并在单剂量给药中刺激骨相关蛋白的表达。通过溶胶-凝胶法制备了SiNPs-CaP纳米材料，并通过合成后功能化方法添加了离子。合成的SiNPs-CaP具有窄的尺寸分布，良好的胶体稳定性，并显示出高水平的离子结合。此外，SiNPs-CaP具有良好的细胞相容性，并促进人骨髓间充质干细胞（hBMSC）的成骨分化，其碱性磷酸酶，骨桥蛋白和骨钙素的生产水平可与标准成骨培养基中的水平相比。新型生物活性SiNPs-CaP以简单，快速的方式合成，显示出单剂量给药后能够促进成骨分化的能力，而与外部成骨诱导剂无关，显示出在骨组织工程应用中作为载体的巨大潜力。

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