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首页> 外文期刊>Materials science & engineering >Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction
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Inflammatory response and macrophage polarization using different physicochemical biomaterials for oral and maxillofacial reconstruction

机译：使用不同理化生物材料进行口腔和颌面重建的炎症反应和巨噬细胞极化

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Knowledge about the action of immune system in the recognition of biomaterials has been extremely helpful when it comes about understanding host response and biomaterials fate in human body. This study aimed to investigate inflammatory response and macrophage polarization during bone healing process of rat's calvaria critical defects using different bone materials in order to evaluate their influence on bone repair and on the quality of the newly formed bone tissue. Eighty male cams Wistar rats underwent surgical procedure for the confectioning of a 5-mm diameter bone defect in their right parietal bone, and divided in four groups (n = 20 each), according the biomaterial: AG - Control, particulate intramembranous autogenous bone graft, HA/TCP - particulate biphasic calcium phosphate with HA/TCP (60/40), DBB - particulate deproteinized bovine bone, VC - particulate bioactive vitrocerarmic. After 3, 7, 21, and 45 days, the specimens were removed and prepared for microcomputed tomography (microCT), light and polarized microscopy, immunohistochemical analysis, and histomorphometry. No significant differences were detected considering percentage of leukocytes among the groups and periods, as well as in relation to immunolabeling for inflammatory (M1) and reparative (M2) macrophages. However, immunolabeling for bone marker indicated a delayed osteoblast differentiation in VC group, resulting in a decrease in mineralized bone matrix parameters in this group, revealed by microCT. In addition, AG and HA/TCP presented a satisfactory bone collagenous content. Despite the distinct origins and physicochemical properties of the tested biomaterials, they presented similar immune-inflammatory responses in the present experimental model, influencing bone-related proteins and bone quality, which must be considered according to their use.
机译：当了解人体的宿主反应和生物材料的命运时，有关免疫系统在识别生物材料方面的作用的知识将非常有帮助。本研究旨在研究使用不同骨材料的大鼠颅盖关键性缺损骨愈合过程中的炎症反应和巨噬细胞极化，以评估其对骨修复和新形成的骨组织质量的影响。八十只雄性雄性Wistar大鼠接受了手术治疗，以修复其右顶骨中直径5毫米的骨缺损，并根据生物材料分为四组（每组n = 20）：AG-对照，颗粒状膜内自体骨移植，HA / TCP-含HA / TCP（60/40）的颗粒状双相磷酸钙，DBB-脱蛋白的牛骨颗粒，VC-具生物活性的体外脑微粒。 3、7、21和45天后，取出标本，准备进行微计算机断层扫描（microCT），光和偏光显微镜，免疫组织化学分析和组织形态测定。考虑到各组和各时期之间白细胞的百分比以及与炎性（M1）和修复性（M2）巨噬细胞的免疫标记有关，没有发现显着差异。然而，通过microCT显示，对骨标记物进行免疫标记表明VC组成骨细胞分化延迟，导致该组中矿化的骨基质参数降低。另外，AG和HA / TCP表现出令人满意的骨胶原含量。尽管受试生物材料的起源和理化特性不同，但它们在本实验模型中仍表现出相似的免疫炎症反应，影响骨相关蛋白和骨质量，必须根据其用途来考虑。

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