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首页> 外文期刊>Materials science & engineering >Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications
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Fabrication and characterization of chitosan based collagen/ gelatin composite scaffolds from big eye snapper Priacanthus hamrur skin for antimicrobial and anti oxidant applications

机译：大眼鲷Priacanthus hamrur皮肤的壳聚糖基胶原/明胶复合支架的制备和表征，用于抗菌和抗氧化剂应用

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摘要

In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanduis humrur. Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers. The scaffolds were evaluated for rheological properties - porosity, apparent density and swelling capacity to assess their mechanical property.Gelatin/chitosan composition shown very high porosity(81.02%) and incorporation of collagen shown higher density in Collagen/gelatin/chitosan scaffolds (0.0522g/cm(3)) and collagen/chitosan scaffolds (0.0468 g/cm(3)).Morphology of the prepared scaffolds were analyzed by Scanning Electron Microscopy which showed reduced pore size of 10 to 20p in Collagen/gelatin/Chitosan composite, 5 to 10 mu in gelatin/chitosan composites and 2-5 mu in collagen/chitosan composites. FITR analysis showed intense peaks ranging 1120 -11267 cm(-1) in the three different scaffolds that are denoted as CH groups. In-vitro antioxidant investigation through DPPH assay showed that the composite 3 in 1 mg/ml concentration exhibited higher antioxidant potential (70%). In contrast, ABTS scavenging assay identified compositel in 1 mg/ml had good antioxidant activity with highest percentage of inhibition (29.5%). The scaffolds were also evaluated for anti microbial properties through disc diffusion assay. The results showed maximum inhibition- 14 mm, 12 mm and 14 nun for 200 mu g of the sample for collagen/chitosan, gelatin/chitosan and collagen/gelatin/chitosan scaffolds respectively towards E.Coli and 20 mm, 20 mm and 24 nun towards S.aureus. The zone of inhibition against E. coil and S. aureus for the three scaffolds was comparatively lower and that could be due to the presence of chitosan. The findings of the study indicates that the bioscaffolds are expected to have wide application in tissue engineering.
机译：在这项研究中，为了从鱼类加工废料中开发用于组织工程的支架，使用从海洋大眼鲷鱼Priacanduis humrur的皮肤中提取的胶原蛋白和明胶的混合物制造了一种层状胶原蛋白/明胶/壳聚糖新型多孔支架。通过壳聚糖的机械纺丝并通过混合胶原蛋白和明胶溶液，然后冷冻干燥和随后的聚合物交联来开发支架。评估支架的流变特性-孔隙率，表观密度和溶胀能力以评估其机械性能。明胶/壳聚糖组合物显示出非常高的孔隙率（81.02％），胶原蛋白/明胶/壳聚糖支架中胶原的掺入显示出更高的密度（0.0522g / cm（3））和胶原蛋白/壳聚糖支架（0.0468 g / cm（3））。通过扫描电子显微镜分析制备的支架的形态，结果显示胶原蛋白/明胶/壳聚糖复合材料的孔径减小了10至20p，5明胶/壳聚糖复合材料的使用量为10亩，胶原蛋白/壳聚糖复合材料的使用量为2-5亩。 FITR分析显示在三个不同的支架（称为CH组）中出现1120 -11267 cm（-1）的强烈峰。通过DPPH测定进行的体外抗氧化剂研究表明，浓度为1 mg / ml的复合材料3表现出较高的抗氧化潜能（70％）。相比之下，ABTS清除试验确定1 mg / ml的复合物具有良好的抗氧化活性，具有最高的抑制率（29.5％）。还通过盘扩散测定法评估了支架的抗微生物特性。结果显示，对于200μg的胶原/壳聚糖，明胶/壳聚糖和胶原/明胶/壳聚糖支架样品，分别对E.Coli和20 mm，20 mm和24 nun的最大抑制作用分别为14 mm，12 mm和14 nun。对金黄色葡萄球菌。这三种支架对线圈和金黄色葡萄球菌的抑制区相对较低，这可能是由于壳聚糖的存在。研究结果表明，生物支架有望在组织工程中得到广泛应用。

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