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Low cost synthesis of reduced graphene oxide using biopolymer for influenza virus sensor

机译:利用生物聚合物低成本合成流感病毒传感器还原氧化石墨烯

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摘要

A biocompatible, cost-effective, and scalable reduced graphene oxide (rGO) film was obtained from shellac using thermal treatment and its structural, chemical, and electrical properties were investigated. This thermally-decomposed rGO (TrGO) film exhibited good crystallinity, low sheet resistance, and high carbon content. TrGO flakes obtained from the film were dispersed and drop cast onto indium tin oxide/glass electrodes to fabricate label-free electrochemical immunosensors for the quantitative detection of the influenza virus H1N1 via electrochemical impedance spectroscopy. These sensors exhibited high stability and reproducibility, both possibly ascribable to the high adhesion of TrGO due to its phenolic-OH moiety; the limits of detection were 26 and 33 plaque-forming units, respectively, in phosphate-buffered saline and diluted saliva. These cost-effective TrGO-based sensors showed great potential as reliable and robust nanomaterial-based biosensors for widespread clinical applications.
机译:使用热处理从紫胶中获得了生物相容,具有成本效益且可扩展的氧化石墨烯(rGO)薄膜,并对其结构,化学和电性能进行了研究。该热分解的rGO(TrGO)膜表现出良好的结晶度,低薄层电阻和高碳含量。将从薄膜中获得的TrGO薄片分散并滴铸到氧化铟锡/玻璃电极上,以制造无标记的电化学免疫传感器,用于通过电化学阻抗谱定量检测H1N1流感病毒。这些传感器表现出很高的稳定性和可重复性,这都可能归因于TrGO的酚羟基部分,因此其粘附性很高。在磷酸盐缓冲液和稀释唾液中的检出限分别为26和33个噬菌斑形成单位。这些具有成本效益的基于TrGO的传感器作为可靠且耐用的基于纳米材料的生物传感器,在广泛的临床应用中显示出巨大的潜力。

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