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首页> 外文期刊>Materials science & engineering >Chitin hydrolysis assisted by cell wall degrading enzymes immobilized of Thichoderma asperellum on totally cinnamoylated D-sorbitol beads
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Chitin hydrolysis assisted by cell wall degrading enzymes immobilized of Thichoderma asperellum on totally cinnamoylated D-sorbitol beads

机译：在完全肉桂化的D-山梨糖醇珠上固定化的米曲霉细胞壁降解酶辅助的几丁质水解

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摘要

In this study, cell wall degrading enzymes produced by Thrichoderma asperellum (TCWDE) were immobilized on totally cinnamoylated D-sorbitol (TCNSO) beads and used for chitin hydrolysis. In order to optimize immobilization efficiency, the reaction time was varied from 2 to 12 h and reactions were conducted in the presence or absence of Na_2SO_4. Immobilized enzymes were analysed concerning to thermal and operational stability. Immobilization in presence of Na_2SO_4 was 54% more efficient than immobilization in absence of salt After optimization, 32% of the total enzyme offered was immobilized, with 100% of bounding efficiency, measured as the relation between protein and enzyme immobilized. Free and TCNSO-TCWDE presented very similar kinetics with maximum hydrolysis reached at 90 min of reaction. Thermal stability of both free and TCNSO-TCWDE was similar, with losses in activity after 55 ℃ Moreover, free and TCNSO-TCWDE retained 100% activity after 3 h incubation at 55 ℃. TCNSO-TCWDE were used in a bath-wise reactor during 14 cycles, producing 1825 μg of N-acetylglucosamine (NAG) maintaining 83% of initial activity.
机译：在这项研究中，由米曲霉（TCWDE）产生的细胞壁降解酶被固定在完全肉桂化的D-山梨醇（TCNSO）珠上，并用于几丁质水解。为了优化固定效率，反应时间从2到12 h不等，反应在有或没有Na_2SO_4的条件下进行。分析固定化酶的热稳定性和操作稳定性。在Na_2SO_4存在下的固定化比在不加盐的情况下固定化的效率高54％。优化后，以蛋白质和固定化酶之间的关系衡量，固定化了32％的总酶，结合效率为100％。游离和TCNSO-TCWDE表现出非常相似的动力学，在反应90分钟时达到最大水解。游离和TCNSO-TCWDE的热稳定性相似，在55℃后活性降低。此外，游离和TCNSO-TCWDE在55℃孵育3 h后仍保持100％活性。 TCNSO-TCWDE在14个循环的浴式反应器中使用，产生1825μgN-乙酰氨基葡萄糖（NAG），保持83％的初始活性。

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  • 来源
    《Materials science & engineering》 |2013年第5期|3077-3081|共5页
  • 作者

    Katia F. Fernandes; David Cortijo-Trivino; Karla A. Batista; Cirano J. Ulhoa; Pedro A. Garcia-Ruiz;

  • 作者单位

    Departamento de Bioqulmica e Biologia Molecular, Instituo de Ciencias Bioldgicas, Universidade Federal de Golas, Cx, Postal 131, 74001-970 Goiania, GO, Brazil,Grupo de Quimica de Carbohidratos y Biotecnologia de Alimentos (QCBA), Departamento de Qulmica Organica, Facultad de Quimica, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain;

    Grupo de Quimica de Carbohidratos y Biotecnologia de Alimentos (QCBA), Departamento de Qulmica Organica, Facultad de Quimica, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain;

    Departamento de Bioqulmica e Biologia Molecular, Instituo de Ciencias Bioldgicas, Universidade Federal de Golas, Cx, Postal 131, 74001-970 Goiania, GO, Brazil;

    Departamento de Bioqulmica e Biologia Molecular, Instituo de Ciencias Bioldgicas, Universidade Federal de Golas, Cx, Postal 131, 74001-970 Goiania, GO, Brazil;

    Grupo de Quimica de Carbohidratos y Biotecnologia de Alimentos (QCBA), Departamento de Qulmica Organica, Facultad de Quimica, Universidad de Murcia, E-30100 Espinardo, Murcia, Spain;

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  • 正文语种 eng
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  • 关键词

    Chitinase; Immobilization; Oligosaccharides; Cinnamoylated D-sorbitol; Reactor; Thrichoderma asperellum;

    机译：几丁质酶;固定;寡糖;肉桂基D-山梨醇;反应堆;曲霉;

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