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首页> 外文期刊>Materials science & engineering >Development of amperometric laccase biosensor through immobilizing enzyme in copper-containing ordered mesoporous carbon (Cu-OMC)/chitosan matrix
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Development of amperometric laccase biosensor through immobilizing enzyme in copper-containing ordered mesoporous carbon (Cu-OMC)/chitosan matrix

机译：通过将酶固定在含铜有序介孔碳（Cu-OMC）/壳聚糖基质中来开发电流型漆酶生物传感器

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摘要

A functionalized copper-containing ordered mesoporous carbon (Cu-OMC) which shows good electrical properties was synthesized by carbonization of sucrose in the presence of cupric acetate inside SBA-15 mesoporous silica template. Based on this, a facilely fabricated amperometric biosensor by entrapping laccase into the Cu-OMC/chitosan (CS) film was developed. Laccase from Trametes versicolor was assembled on a composite film of Cu-OMC/chitosan (CS) modified Au electrode and the electrode was characterized. The optimum experimental conditions of biosensor for the detection of catechol were studied in details. Under the optimal conditions, the detection limit was 0.67 μM and the linear detection range was from 0.67 μM to 15.75 μM for catechol. The apparent Michaelis-Menten (K_M~(app)) was estimated using the Lineweaver-Burk equation and the K_M~(app) value was 40.2 μM. This work demonstrated that the Cu-OMC/CS composite provides a suitable support for laccase immobilization and construction of biosensor.
机译：通过在SBA-15介孔二氧化硅模板内部存在乙酸铜的条件下，将蔗糖碳化来合成具有良好电性能的功能化的含铜有序介孔碳（Cu-OMC）。基于此，开发了一种通过将漆酶包埋在Cu-OMC /壳聚糖（CS）膜中而容易制造的安培生物传感器。将来自Trametes versicolor的漆酶组装在Cu-OMC /壳聚糖（CS）改性Au电极的复合膜上，并对电极进行了表征。详细研究了检测邻苯二酚的生物传感器的最佳实验条件。在最佳条件下，邻苯二酚的检出限为0.67μM，线性检测范围为0.67μM至15.75μM。使用Lineweaver-Burk方程估算表观Michaelis-Menten（K_M〜（app）），并且K_M〜（app）值为40.2μM。这项工作表明，Cu-OMC / CS复合材料为漆酶的固定和生物传感器的构建提供了合适的支持。

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  • 来源
    《Materials science & engineering》 |2010年第5期|p.722-729|共8页
  • 作者

    Xinhua Xu; Meiqing Guo; Ping Lu; Rui Wang;

  • 作者单位

    School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China Tianjin Key Laboratory of Composite and Functional Materials, Tianjin 300072, PR China;

    School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China Tianjin Key Laboratory of Composite and Functional Materials, Tianjin 300072, PR China;

    School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China Tianjin Key Laboratory of Composite and Functional Materials, Tianjin 300072, PR China;

    School of Materials Science and Engineering, Tianjin University, Tianjin 300072, PR China Tianjin Key Laboratory of Composite and Functional Materials, Tianjin 300072, PR China;

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  • 正文语种 eng
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  • 关键词

    biosensor; laccase; Cu-OMC; chitosan; electrical properties;

    机译：生物传感器漆酶铜-OMC;壳聚糖电性能;

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