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首页> 外文期刊>Materials science & engineering >Bioactive nanoparticle reinforced alginate/gelatin bioink for the maintenance of stem cell sternness
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Bioactive nanoparticle reinforced alginate/gelatin bioink for the maintenance of stem cell sternness

机译：生物活性纳米粒子增强藻酸盐/明胶生物链，用于维持干细胞沉默

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摘要

Mesenchymal cells (MSCs) are an attractive option as seed cells for bioprinting. However, loss of stemness and undesired differentiation reduces their effectiveness. In this study, 12 nm bioactive nanoparticles (BNPs) which could release silicon (Si) ions were used to enhance the properties of alginate/gelatin hydrogel bioink to maintain MSC stemness. By specifically leveraging biochemical signals of BNPs, bioink with defined stiffness towards osteogenic and adipogenic potential, independent of pore structure, were designed by incorporating with different concentration of BNPs. These bioink were characterized by printability, mechanical and rheological properties as well as osteogenic and adipogenic potentials. Notably, the effect of 2% BNPs addition in alginate/gelatin hydrogel on MSC stemness maintenance was confirmed by the expression of stemness markers. At higher concentrations of BNPs (5%), printability was impacted by the gelling process. We further confirmed the enhanced stemness maintenance by sweat gland lineage commitment of bioprinted MSCs in vitro. Overall, our study proved that alginate/gelatin hydrogel bioink reinforced by BNPs in the optimal concentrations could retain MSC stemness as well as support MSC growth and prolong the desired differentiation. These findings may provide a new approach to achieve the ideal therapeutic potential of MSCs in 3D bioprinting application.
机译：间充质细胞（MSCs）是作为生物印刷制品的种子细胞的有吸引力的选择。然而，茎丧失和不期望的分化降低了它们的有效性。在该研究中，可以利用释放硅（Si）离子的12nM生物活性纳米颗粒（BNP）来增强藻酸盐/明胶水凝胶生物凝胶的性能以维持MSC茎。通过专门利用BNP的生物化学信号，通过掺入不同的BNP浓度，设计了与孔隙结构无关的具有定义刚度朝向成骨和脂肪发生潜力的生物链接。这些生物链的特征在于可印刷性，机械和流变性能以及骨质发生和脂肪发生势。值得注意的是，通过表达茎标记物证实了2％BNP添加到MSC茎固体凝胶上的2％BNPS的作用。在较高浓度的BNPS（5％），可印刷性受到胶凝过程的影响。我们进一步证实了在体外汗腺血管谱系对生物印刷MSCs的汗腺谱系的提高。总体而言，我们的研究证明，在最佳浓度下通过BNP加固的海藻酸盐/明胶水凝胶生物胶体可以保留MSC茎，并且支持MSC生长并延长所需的分化。这些发现可以提供一种新方法来实现3D生物印刷机应用中MSC的理想治疗潜力。

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  • 来源
    《Materials science & engineering》 |2021年第7期|112193.1-112193.11|共11页
  • 作者

    Li Jianjun; Zhang Yijie; Enhe Jirigala; Yao Bin; Wang Yuzhen; Zhu Dongzhen; Li Zhao; Song Wei; Duan Xianlan; Yuan Xingyu; Fu Xiaobing; Huang Sha;

  • 作者单位

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Dept Gen Surg Med Ctr 1 28 Fu Xing Rd Beijing 100853 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China|Tianjin Med Univ Coll Grad 22 Qi Xiang Tai Rd Tianjin 300050 Peoples R China|Inner Mongolia Med Univ Inst Basic Med Res Hohhot Inner Mongolia Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China|Air Force Hosp Chinese PLA Cent Theater Command Dept Burn & Plast Surg 589 Yun Zhong Rd Datong 037006 Shanxi Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China|Nankai Univ Sch Med 94 Wei Jing Rd Tianjin 300071 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China|Nankai Univ Sch Med 94 Wei Jing Rd Tianjin 300071 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp PLA Key Lab Tissue Repair & Regenerat Med 51 Fu Cheng Rd Beijing 100048 Peoples R China|Chinese Peoples Liberat Army Gen Hosp Beijing Key Res Lab Skin Injury Repair & Regenera 51 Fu Cheng Rd Beijing 100048 Peoples R China|PLA Med Coll 51 Fu Cheng Rd Beijing 100048 Peoples R China;

    Peoples Liberat Army Gen Hosp Res Ctr Tissue Repair & Regenerat Med Innovat Res Dept 51 Fu Cheng Rd Beijing 100048 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Bioink; Bioactive nanoparticles; Stemness; Silicon; Bioprinting;

    机译：生物链;生物活性纳米粒子;茎;硅;生物印刷;

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