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首页> 外文期刊>Meat Science >Identification of hare meat by a species-specific marker of mitochondrial origin
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Identification of hare meat by a species-specific marker of mitochondrial origin

机译：通过线粒体起源的物种特异性标记鉴定野兔肉

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摘要

Meat species identification in food has gained increasing interest in recent years due to public health, economic and legal concerns. Following the consumer trend towards high quality products, game meat has earned much attention. The aim of the present work was to develop a DNA-based technique able to identify hare meat. Mitochondrial cytochrome b gene was used to design species-specific primers for hare detection. The new primers proved to be highly specific to Lepus species, allowing the detection of 0.01 % of hare meat in pork meat by polymerase chain reaction (PCR). A real-time PCR assay with the new intercalating EvaGreen dye was further proposed as a specific and fast tool for hare identification with increased sensitivity (1 pg) compared to end-point PCR (10 pg). It can be concluded that the proposed new primers can be used by both species-specific end-point PCR or real-time PCR to accurately authenticate hare meat.
机译：近年来，由于公众健康，经济和法律方面的考虑，食品中肉类的识别越来越引起人们的关注。随着消费者趋向于高质量产品的趋势，野味受到了广泛的关注。本工作的目的是开发一种能够识别野兔肉的基于DNA的技术。线粒体细胞色素b基因用于设计物种特异性引物，用于野兔检测。事实证明，新引物对狼疮物种具有高度特异性，可通过聚合酶链反应（PCR）检测猪肉中0.01％的野兔肉。与终点PCR（10 pg）相比，进一步提出了一种使用新型插层式EvaGreen染料的实时PCR分析法，作为特异性更高的兔子鉴定方法，灵敏度更高（1 pg）。可以得出结论，建议的新引物可用于物种特异性终点PCR或实时PCR来准确鉴定野兔肉。

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  • 来源
    《Meat Science》 |2012年第3期|p.836-841|共6页
  • 作者

    Cristina G. Santos; Vitor S. Melo; Joana S. Amaral; Leticia Estevinho; M. Beatriz P.P. Oliveira; Isabel Mafra;

  • 作者单位

    REQUIMTE, Departamento de Ciencias Quimicas, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha, 164, 4099-030 Porto, Portugal,Institute Polkecnico de Bragartfa, Campus de Sta. Apolonia, 5301-857 Braganga, Portugal;

    REQUIMTE, Departamento de Ciencias Quimicas, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha, 164, 4099-030 Porto, Portugal,Institute Polkecnico de Bragartfa, Campus de Sta. Apolonia, 5301-857 Braganga, Portugal;

    REQUIMTE, Departamento de Ciencias Quimicas, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha, 164, 4099-030 Porto, Portugal,Institute Polkecnico de Bragartfa, Campus de Sta. Apolonia, 5301-857 Braganga, Portugal;

    Institute Polkecnico de Bragartfa, Campus de Sta. Apolonia, 5301-857 Braganga, Portugal;

    REQUIMTE, Departamento de Ciencias Quimicas, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha, 164, 4099-030 Porto, Portugal;

    REQUIMTE, Departamento de Ciencias Quimicas, Faculdade de Farmacia, Universidade do Porto, Rua Anibal Cunha, 164, 4099-030 Porto, Portugal;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    hare meat; lepus; species identification; authenticity; real-time PCR; evagreen;

    机译：野兔肉狼疮物种鉴定;真实性实时PCR;淡绿色;

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