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首页> 外文期刊>Meat Science >Determination of residual ractopamine concentrations by enzyme immunoassay in treated pig's tissues on days after withdrawal
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Determination of residual ractopamine concentrations by enzyme immunoassay in treated pig's tissues on days after withdrawal

机译：停药后几天，通过酶联免疫法测定处理过的猪组织中残留的莱克多巴胺浓度

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摘要

The objective of this study was to measure residual ractopamine concentrations in tissues of pigs as experimental animals after treatment with dietary ractopamine for 28 consecutive days. Ractopamine was administered orally to the experimental group (n = 9) in a dose of 0.1 mg/kg body mass per day, whereas control animals (n = 3) were left untreated. Treated pigs (60 kg) were sacrificed on days 1,3 and 8 of treatment discontinuation and residues were determined in kidney, liver, muscle, brain and heart tissues using previously validated enzyme-linked immunosorbent assay (ELISA) as a quantitative screening method. Validation showed good mean recoveries (approx. 70-90%) with acceptable inter- and intra-day relative standard deviations (RSD<13%), demonstrating the method efficiency in determination of ractopamine tissue concentrations. The highest ractopamine concentration on day 1 (24 h) after the last exposure was recorded in the kidney (12.49± 7.96 ng/g), followed by the liver (7.21 ±2.73 ng/g), heart (1.26 ± 0.12 ng/g) and brain (0.63 ±0.05 ng/g); at this time of withdrawal, residues were not detected in the muscle. Ractopamine was depleted rapidly from all study tissues, with mostly no detectable residues on day 8 of withdrawal.
机译：这项研究的目的是在连续28天用饮食中的莱克多巴胺治疗后，测量作为实验动物的猪组织中残留的莱克多巴胺浓度。莱克多巴胺以每天0.1 mg / kg体重的剂量口服给予实验组（n = 9），而对照组动物（n = 3）则未经治疗。在治疗中断的第1、3和8天处死被处理的猪（60公斤），并使用先前验证的酶联免疫吸附测定（ELISA）作为定量筛选方法确定肾脏，肝脏，肌肉，脑和心脏组织中的残留。验证显示良好的平均回收率（约70-90％），日间和日间相对标准偏差（RSD <13％）可以接受，证明了该方法在测定莱克多巴胺组织浓度中的效率。最后一次暴露后第1天（24 h）的最高莱克多巴胺浓度记录在肾脏（12.49±7.96 ng / g），然后是肝脏（7.21±2.73 ng / g），心脏（1.26±0.12 ng / g） ）和脑（0.63±0.05 ng / g）;在停药时，未在肌肉中发现残留物。莱克多巴胺迅速从所有研究组织中耗竭，停药后第8天几乎没有可检测到的残留物。

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  • 来源
    《Meat Science》 |2012年第3期|p.755-758|共4页
  • 作者

    Jelka Pleadin; Nina Persi; Ana Vulic; Dinka Milic; Nada Vahcic;

  • 作者单位

    Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR- 10 000 Zagreb, Croatia;

    Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR- 10 000 Zagreb, Croatia;

    Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, HR- 10 000 Zagreb, Croatia;

    Dubravica Swine Farm, Ltd., Pavla Stoosa 109, HR- 10 293 Dubravica, Croatia;

    Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR- 10 000 Zagreb, Croatia;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    ractopamine; residues; ELISA; pig; tissues;

    机译：莱克多巴胺残留物;ELISA;猪;组织;

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