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首页> 外文期刊>Meat Science >Presence of ochratoxin A on the surface of dry-cured Iberian ham after initial fungal growth in the drying stage
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Presence of ochratoxin A on the surface of dry-cured Iberian ham after initial fungal growth in the drying stage

机译：在干燥阶段初期真菌生长后，干腌伊比利亚火腿表面存在of曲霉毒素A

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摘要

Accumulation of ochratoxin A (OTA) on the surface and to a 0.5 cm depth of dry-cured Iberian ham after initial fungal growth was investigated. For this, 20 dry-cured Iberian hams from the drying stage showing incipient fungal growth on the surface were analyzed. In addition, the presence of OTA-producing molds was examined on the surface of the hams by real-time quantitative PCR (qPCR) based on the otanpsPN gene. Quantification of specific OTA-producing molds, such as Penicillium nordicum and Penicillium verrucosum was also achieved on the hams by specific qPCR methods. Ten of 20 dry-cured hams showed OTA at higher levels than those established by legal regulation. OTA was even detected in the deep section of hams. OTA-producing molds ranged from 1.5 to 7.3 log cfu/cm~2. Accumulation of OTA on the hams seems to be related to the presence of OTA-producing molds and especially to P. nordicum.
机译：初始真菌生长后，研究了曲霉毒素A（OTA）在表面和干腌伊比利亚火腿0.5 cm深度的积累。为此，分析了从干燥阶段开始的20只干腌伊比利亚火腿，这些火腿在表面开始出现真菌生长。此外，通过基于otanpsPN基因的实时定量PCR（qPCR）检查了火腿表面是否存在产生OTA的霉菌。还可以通过特定的qPCR方法对火腿中的特定OTA产霉菌进行定量，例如诺迪氏青霉和疣状青霉。 20个干腌火腿中有10个显示的OTA含量高于法律规定的水平。甚至在火腿深部也检测到OTA。生产OTA的模具的范围为1.5至7.3 log cfu / cm〜2。火腿中OTA的积累似乎与生产OTA的霉菌有关，特别是与北欧假单胞菌有关。

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  • 来源
    《Meat Science》 |2012年第4期|p.728-734|共7页
  • 作者

    Alicia Rodriguez; Mar Rodriguez; Alberto Martin; Josue Delgado; Juan J. Cordoba;

  • 作者单位

    Food Hygiene and Safety, Faculty of Veterinary Science, University of Extremadura, Avda. de la Universidad, s. 10003-Caceres, Spain;

    Food Hygiene and Safety, Faculty of Veterinary Science, University of Extremadura, Avda. de la Universidad, s. 10003-Caceres, Spain;

    Bromatology and Nutricion, Agricultural Engineering School, University of Extremadura, Badajoz, Spain;

    Food Hygiene and Safety, Faculty of Veterinary Science, University of Extremadura, Avda. de la Universidad, s. 10003-Caceres, Spain;

    Food Hygiene and Safety, Faculty of Veterinary Science, University of Extremadura, Avda. de la Universidad, s. 10003-Caceres, Spain;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    OTA; dry-cured ham; mold; qPCR;

    机译：OTA;干腌火腿;模子;定量PCR;

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