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A Review of Automated Microinjection Systems for Single Cells in the Embryogenesis Stage

机译:胚胎发生阶段单细胞自动显微注射系统的综述

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摘要

Modern genetics research has resulted in significant advances in cell in vitro microinjection systems. Such systems provide biological and medical practitioners with high volume cell throughput and statistically relevant data. This paper provides the reader with a comprehensive review of the major research technologies used in automated cell microinjection and of their individual subsystems. Microinjection subsystems reviewed include machine vision, nonvision sensors and user interface (input), cell modeling, piercing mechanisms and injection control loop (control), cell holder and manipulator, and microinjection (output). The interdisciplinary technologies reviewed for microinjection sensing, automation, and control include microfluidic actuation, optical field actuation (optical trapping and optical guidance), electrical field actuation (electrorotation, electrowetting, and dielectrophoresis), and ultrasonic vibration. The survey concludes that research into automated microinjection systems will focus on reducing the scale of microinjection systems and developing appropriate controllers.
机译:现代遗传学研究已在细胞体外显微注射系统中取得了重大进展。这样的系统为生物学和医学从业者提供高体积的细胞通量和统计上相关的数据。本文为读者提供了对自动细胞显微注射中使用的主要研究技术及其各个子系统的全面综述。审查的微注射子系统包括机器视觉,非视觉传感器和用户界面(输入),细胞建模,穿刺机制和注射控制环(控制),细胞固定器和操纵器以及微注射(输出)。审查的关于微注射传感,自动化和控制的跨学科技术包括微流体致动,光场致动(光学陷波和光导),电场致动(电旋转,电润湿和介电泳)和超声振动。该调查得出的结论是,对自动显微注射系统的研究将集中在减小显微注射系统的规模和开发合适的控制器上。

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