• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mechatronics, IEEE/ASME Transactions on >A High-Throughput Automated Microinjection System for Human Cells With Small Size
【24h】

A High-Throughput Automated Microinjection System for Human Cells With Small Size

机译：用于小尺寸人体细胞的高通量自动显微注射系统

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This paper presents the development of an automated microinjection system with high productivity for small cells. Compared with many existing microinjection systems that are primarily designed for relatively large-scaled biological samples, the reported technology will enable an automated injection into the cells with a diameter smaller than 25 μm, which is the typical size of many human cells. Microfluidic technology has been used to design a vacuum-based cell-holding device for immobilizing cells. The cell holder employs hundreds of regular-shaped channels, and each channel is occupied by one cell. The vision-based position-tracking method is used to recognize and position the target cells automatically, in which the cell position is measured by edge template-matching method, and a bright field image is used to simplify the recognition process. A 3-DOF microrobotic system mounted with a micropipette is used to conduct cell injection task with the speed of 35 cells/min and the precision of 0.2 μm. Injection experiments on human foreskin fibroblast and human embryonic stem cell-derived ventricular cardiomyocyte were performed to demonstrate the effectiveness of the developed system. More than 1000 cells were injected with fluorescent markers. Experimental results showed that the developed injection system exhibits high productivity and accuracy, and results in a good cell survival rate after injection.
机译：本文介绍了一种自动化的显微注射系统的开发，该系统对小细胞具有高生产率。与主要为相对大规模的生物样品设计的许多现有显微注射系统相比，该报道的技术将能够自动注射直径小于25μm的细胞，这是许多人类细胞的典型大小。微流体技术已被用于设计用于固定细胞的基于真空的细胞固定装置。单元格支架使用数百个规则形状的通道，每个通道都由一个单元格占据。基于视觉的位置跟踪方法用于自动识别和定位目标细胞，通过边缘模板匹配法测量细胞位置，采用明场图像简化识别过程。使用装有微量移液器的3-DOF微型机器人系统以35个细胞/分钟的速度和0.2μm的精度执行细胞注射任务。对人包皮成纤维细胞和人胚胎干细胞衍生的心室心肌细胞进行了注射实验，以证明所开发系统的有效性。用荧光标记物注射了1000多个细胞。实验结果表明，所开发的注射系统具有较高的生产率和准确性，并在注射后具有良好的细胞存活率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号