首页>
外国专利>
High-Throughput Organ-Targeted Microinjection System
High-Throughput Organ-Targeted Microinjection System
展开▼
机译：高通量器官靶向显微注射系统
展开▼
页面导航
摘要
著录项
相似文献
摘要
Automatic system for efficient delivery of biologics into target organs of zebrafish larvae for high-throughput in vivo screening. The system includes a reservoir containing zebrafish larvae immersed in a hydrogel in its liquid state. A microfluidic component removes a droplet of the hydrogel having a single zebrafish larva contained therein and deposits the droplet on a surface for receiving an array of hydrogel droplets. Structure or substances is provided for inducing the larva to assume a dorsal or lateral orientation within the droplet. A cooler cools the surface to solidify the hydrogel droplets thereby to immobilize the larvae for observation by an optical arrangement that identifies target organs in each larva using an image template-matching algorithm. A pressure driven microinjection needle injects biologics into the target organ of the zebrafish larva for screening studies.
展开▼
